連続脳切片を用いた神経突起の三次元再構築のためのステ-ジ連動型描画装置の試作

为了加快单神经元三维结构的分析速度，研制了一种新的重建装置。该装置由带摄像装置的普通显微镜、电动显微镜工作台和可沿X、Y方向移动的电动工作台组成。电动工作台通过微处理器系统与电动工作台的移动成比例地移动，使得显微镜下的脑切片上所需的结构可以通过绘图附件来绘制，然后，通过将邻近的结构移到显微镜视野之外的位置到观察者手中，这些结构可以被连续地描述。通过重复这个过程，一个切片上的单个神经元的整个结构可以被追踪到。序列节的处理方式是相同的。最后，逐一调整脑切片的每一张图，将其中一张切片的神经元结构的断端连接到前一张切片上的适当断端。这种设备可以追踪神经元结构，并将其放大到1.5米宽x 2米长。重建时间加快了三倍。

To accelerate the speed of analysis on three dimensional structures of single neurons, a new reconstruction device was developed. This device was composed of a conventional microscope with a camera lucida drawing attachment, a motor-driven microscope stage and a motor-driven table which can be moved in X Y directions. The motor-driven tables moves in proportion to the movement of the motor-driven stage by a microprocessor system, so that the desired structures on the brain slice under a microscope can be drawn through the drawing attachment and then, by moving the neighboring structures just outside the view of the microscope in to the viewer, those structures can be described continuously. By repeating this procedure, the whole structure of a single neuron on one slice can be traced. Serial sections are processed in the same way. Finally, each drawing of one brain section adjusted one by one to connect cut ends of neuronal structures of one slice to the appropriate cut ends on the previous slice. This device can trace a neuronal structure and enlarge it to 1.5m wide x 2 m long. The reconstruction time is speeded by three times.