連続脳切片を用いた神経突起の三次元再構築のためのステ-ジ連動型描画装置の試作

使用连续脑切片三维重建神经突的阶段联动绘图装置原型

• 批准号: 63870008
• 负责人: SHINODA Yoshikazu
• 金额: $ 4.16万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63870008/
• 关键词: Reconstruction device; Neuron; Microscope; Golgi staining; HRP staining; 形態解析; 三次元再構築; 三次元コンピュ-タ-解析描画装置; 三次元コンピュ-ター解析描画装置; HRP; 前庭核; 脊髄; 小脳; 細胞内染色

To accelerate the speed of analysis on three dimensional structures of single neurons, a new reconstruction device was developed. This device was composed of a conventional microscope with a camera lucida drawing attachment, a motor-driven microscope stage and a motor-driven table which can be moved in X Y directions. The motor-driven tables moves in proportion to the movement of the motor-driven stage by a microprocessor system, so that the desired structures on the brain slice under a microscope can be drawn through the drawing attachment and then, by moving the neighboring structures just outside the view of the microscope in to the viewer, those structures can be described continuously. By repeating this procedure, the whole structure of a single neuron on one slice can be traced. Serial sections are processed in the same way. Finally, each drawing of one brain section adjusted one by one to connect cut ends of neuronal structures of one slice to the appropriate cut ends on the previous slice. This device can trace a neuronal structure and enlarge it to 1.5m wide x 2 m long. The reconstruction time is speeded by three times.

为了加快对单个神经元三维结构的分析速度，研制了一种新的重建装置。该装置由普通显微镜、电机驱动显微镜台和可在X - Y方向移动的电机驱动显微镜台组成。由微处理器系统驱动的马达驱动工作台与马达驱动工作台的运动成比例地移动，这样就可以通过绘图附件在显微镜下绘制出大脑切片上所需的结构，然后通过将显微镜视野外的邻近结构移动到观察者中，这些结构就可以连续地描述出来。通过重复这个过程，单个神经元在一个切片上的整个结构可以被追踪。串行段以同样的方式处理。最后，每张脑切片图逐一调整，将某一切片神经元结构的切割端与前一切片上相应的切割端连接起来。该装置可以追踪神经元结构，并将其放大到1.5米宽× 2米长。重建时间加快了三倍。

项目成果

Shinoda, Y.: "Vestibular projections to the spinal cord. In "Vestibular control of posture and movement."" Prog. in Brain Res. Vol.76 (17-27) 1988.

Shinoda, Y.：“前庭对脊髓的投射。在“姿势和运动的前庭控制”中。”Prog。

Shinoda,Y.,S.Kakei: "Distribution of terminals of thalamocortical fibers originating from the ventrolateral nucleus of the cat thalamus" Neuroscience Letters. 96. 163-167 (1989)

Shinoda，Y.，S.Kakei：“源自猫丘脑腹外侧核的丘脑皮质纤维末端的分布”神经科学快报。

篠田義一: "新生理学大系8神経生理学総論破壊と刺激" 伊藤正男,塚原仲晃, 132-150 (1989)

筱田义一：《新生理学系统8神经生理学通论破坏与刺激》Masao Ito，Nakaaki Tsukahara，132-150（1989）

Shinoda,Y.et al.: "Spatial innervation patterns of single vestibulospinal axons in neck motor nuclei" Head-Neck Sensory-Motor System Berthoz,A.(Ed),IBRO Symposium. (1990)

Shinoda,Y.et al.：“颈部运动核中单个前庭脊髓轴突的空间神经支配模式”头颈感觉运动系统 Berthoz,A.（编辑），IBRO 研讨会。

Shinoda,Y.: Progress in Brain Research. 76. 17-27 (1988)

Shinoda，Y.：大脑研究进展。