权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Developing a new computer-assisted data adquisition and analysis systems for in vivo electrophysiological experiments in the mamalian central nervous system

开发用于哺乳动物中枢神经系统体内电生理实验的新型计算机辅助数据采集和分析系统

基本信息

批准号：
07558292
负责人：
SHINODA Yoshikazu
金额：
$ 0.45万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

This study was aimed at developing a computer-assisted recording system for electrophysiological data in in vivo experiments of the mammalian central nervous system. In an electrophysiological experiment of the mammalian central nervous system, we stimulate a certain structure, record electrical potentials from inside a neuron and analyze the neural connections between them. Intracellular recording is very difficult and it is much harder to keep recording from a cell for a long time. To record as much data as possible for a limited period, many researchers use a data recorder to acquire the data. This is convenient for off-line data analysis. On the other hand, we also have to record various parameters which are used for stimulation and recording. These values are usually written down on a notebook or recorded on a voice channel on a tape. For later analysis of the data on a computer, these parameters were typed from a key board. It takes a long time for acquiring the data into the computer and also typing the necessary parameters. Therefore, it is desirable that we will develop a new recording system for neurophysiological experiments which wll speed up recording and analytical procedures. There are several commercially available computer-assisted system for neurophysiological data acquisition and analysis. However, these systems are only useful for patch clamp experiments or unit recording in chronic electrophysiological experiments because of their slow data acquisition capability. We made a new stimulator which could tell the computer all necessary stimulus parameters by digital signals. Computer software was developed to acquire data, and display and store data simultaneously at high frequencies. With this new stimulating and recording systems, we speed up the recording procedure and get more data for a limited period. Furthermore, it is much easier and faster to analyze data on a computer.

本研究的目的是开发一个计算机辅助记录系统，用于哺乳动物中枢神经系统在体实验中的电生理数据。在哺乳动物中枢神经系统的电生理学实验中，我们刺激某个结构，记录神经元内部的电位，并分析它们之间的神经连接。细胞内记录是非常困难的，并且很难长时间保持从细胞记录。为了在有限的时间内记录尽可能多的数据，许多研究人员使用数据记录仪来获取数据。这便于离线数据分析。另一方面，我们还必须记录用于刺激和记录的各种参数。这些值通常记在笔记本上或记录在磁带的语音通道上。为了以后在计算机上分析数据，这些参数是从键盘上输入的。将数据采集到计算机中并输入必要的参数需要很长时间。因此，我们希望开发一种新的记录系统，用于神经生理学实验，以加快记录和分析过程。有几种商业上可获得的用于神经生理数据采集和分析的计算机辅助系统。然而，这些系统仅适用于膜片钳实验或慢性电生理实验中的单位记录，因为它们的数据采集能力慢。我们研制了一种新的刺激器，它能以数字信号的形式将所需的刺激参数告诉计算机。开发了计算机软件，以高频采集数据，并同时显示和存储数据。有了这种新的刺激和记录系统，我们加快了记录过程，并在有限的时间内获得更多的数据。此外，在计算机上分析数据更容易和更快。