The protist microbiota of maize and their functional roles for the self-organization of the rhizosphere bacterial microbiome (ProtistBiome II)

该项目的目的是表征玉米根际与植物相关的原生生物群落。我们将根际群落理解为动态系统，并希望研究土壤理化性质（质地）和根系活性、基因表达和渗出的变化如何影响土传微生物群落。微生物和它们的捕食者与根系共同进化。它们的包囊和休眠阶段，特别是病原体，在土壤中积累并影响后代植物的微生物群和根系。重点是尾虫和卵菌，它们是具有不同植物相关分类群的优势和物种原生生物群体，我们为此开发了群体特异性引物。这些分析伴随着宏基因组分析来量化细菌组成和功能的变化。

The aim of the project is to characterise the plant-associated protist community in the rhizosphere of maize. We understand rhizosphere communities as dynamic systems and want to investigate how physico-chemical soil properties (texture) and changes in root activity, gene expression and exudation influence soil-borne microbial communities. Microorganisms and their predators co-evolve with the root system. Their cysts and resting stages, especially pathogens, accumulate in the soil and affect the microbiome and root system of subsequent plant generations. The focus is on cercozoans and oomycetes, which are dominant and speciose protist groups with distinct plant-associated taxa, for which we have developed group-specific primers. The analyses are accompanied by metagenome analyses to quantify changes in bacterial composition and function.