Continuing the deep molecular characterization of eukaryotic microorganisms´ diversity and community composition in forest soils and the canopy region using a metatranscriptomics approach (micDiv II)

使用元转录组学方法继续对森林土壤和冠层区域的真核微生物多样性和群落组成进行深入的分子表征（micDiv II）

• 批准号: 447013012
• 负责人: Professor Dr. Michael Bonkowski
• 金额: --
• 依托单位: AG Molekulare Evolution und Systematik der Tiere
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/447013012?language=en
• 关键词: Continuing; deep; molecular; characterization; eukaryotic

Tree canopies provide habitats for extremely diverse and until now, still poorly characterized communities of microbial eukaryotes (Protists). This is the major result of our first project phase. High-throughput sequencing of the two protist phyla Oomycetes (Stramenopiles) and Cercozoa (Rhizaria) revealed on average > 400 and > 700 OTUs, respectively. Additionally, we assessed in a first study the species richness and abundances of protists in air samples and found a high diversity of passively dispersed taxa. Subsequent targeted taxonomic studies showed that the genus Rhogostoma was prominant in air samples, tree crowns and leaf surfaces, respectively. Only five Rhogostoma species have been described to date, but we detected 23 new clades (!) in the genus Rhogostoma. We aim to quantify the mechanisms leading to the high diversity of protists in tree canopies such as neutral community assembly from passive dispersal and deterministic, niche-based processes.

树冠提供栖息地极其多样化，直到现在，仍然缺乏特色的社区微生物真核生物（原生生物）。这是我们项目第一阶段的主要成果。两个原生动物门卵菌纲（Stratifiles）和尾藻纲（Rhizaria）的高通量测序分别显示平均> 400和> 700 OTU。此外，我们在第一项研究中评估了空气样品中原生生物的物种丰富度和丰度，发现了被动分散类群的高度多样性。随后的定向分类学研究表明，Rhogostoma属分别在空气样品、树冠和树叶表面上是透明的。到目前为止，只有五种Rhogostoma物种被描述，但我们发现了23个新的分支（！）在Rhogostoma属中。我们的目标是量化的机制，导致高多样性的原生生物在树冠层，如中性社区组装从被动扩散和确定性，生态位为基础的过程。