Role of extracellular vesicles as communication mediators between pathogens to potentiate pathogenicity in wound infections

细胞外囊泡作为病原体之间的通讯介质，增强伤口感染的致病性

• 批准号: 22K15454
• 负责人: Subsomwong Phawinee
• 金额: $ 2.58万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K15454/
• 关键词: Staphylococcus aureus; Pseudomonas aeruginosa; Extracellular vesicles; Extracellular vesicle; Interaction; Pathogenicity

The co-infection of S. aureus and P. aeruginosa is common in patients with chronic wounds, but little is known about their synergistic effect mediated by extracellular vesicles (EVs). In this study, we investigate the effect of EVs derived from S. aureus (SaEVs) and P. aeruginosa (PaEVs) on the pathogenicity of their bacterial counterparts. In FY2022, we succeeded in the isolation and purification of the EVs from both pathogens. The effects of SaEVs, and PaEVs on their bacterial counterparts included growth, antibiotic resistance, biofilm formation, and pathogenicity were performed. We found that PaEVs inhibited the growth of S. aureus. In contrast, SaEVs did not have an effect on the growth of P. aeruginosa. Because of PaEVs inhibited S. aureus growth, the following experiments such as biofilm formation, antibiotic resistance, and cell infection were not investigated. We found SaEVs did not change the antibiotic-resistance pattern of P. aeruginosa. Interestingly, P. aeruginosa-treated with SaEVs had higher biofilm formation than non-treated P. aeruginosa with SaEVs. In addition, the SaEVs enhanced the human keratinocyte cell line’s invasion of P. aeruginosa. In a further study, we will investigate the molecular mechanisms of SaEVs to promote P. aeruginosa biofilm formation and host cell invasion. Furthermore, the mechanism by which PaEVs inhibit S. aureus will investigate.

S.金黄色葡萄球菌和铜绿假单胞菌在慢性伤口患者中很常见，但对它们由细胞外囊泡（EV）介导的协同作用知之甚少。在这项研究中，我们研究了来自S.金黄色葡萄球菌（SaEV）和铜绿假单胞菌（PaEV）对它们的细菌对应物的致病性的影响。于二零二二财政年度，我们成功从两种病原体中分离及纯化EV。进行SaEV和PaEV对其细菌对应物的影响，包括生长、抗生素抗性、生物膜形成和致病性。我们发现PaEVs抑制了S.金黄色。相反，SaEV对铜绿假单胞菌的生长没有影响。由于PaEVs抑制S.金黄色葡萄球菌生长，未研究以下实验，例如生物膜形成、抗生素抗性和细胞感染。我们发现SaEV并没有改变铜绿假单胞菌的耐药模式。有趣的是，用SaEV处理的铜绿假单胞菌比用SaEV未处理的铜绿假单胞菌具有更高的生物膜形成。此外，SaEV增强了人角质形成细胞系对铜绿假单胞菌的侵袭。在进一步的研究中，我们将研究SaEV促进铜绿假单胞菌生物膜形成和宿主细胞侵袭的分子机制。此外，PaEV抑制S.奥利亚会调查的

项目成果

Extracellular vesicles of Staphylococcus aureus promote exopolysaccharide biosynthesis and pathogenicity of Pseudomonas aeruginosa

金黄色葡萄球菌胞外囊泡促进胞外多糖生物合成及铜绿假单胞菌致病性

• 发表时间: 2022
• 作者: Subsomwong Phawinee;Doohan Dalla;Fauzia Kartika Afrida;Akada Junko;Matsumoto Takashi;Yee Than Than;Htet Kyaw;Waskito Langgeng Agung;Tuan Vo Phuoc;Uchida Tomohisa;Matsuhisa Takeshi;Yamaoka Yoshio;Subsomwong Phawinee;Subsomwong Phawinee;Subsomwong Phawinee;Subsomwong Phawinee
• 通讯作者: Subsomwong Phawinee

Extracellular vesicles of Staphylococcus aureus promote pathogenicity of Pseudomonas aeruginosa

金黄色葡萄球菌胞外囊泡促进铜绿假单胞菌的致病性

• 发表时间: 2023
• 作者: Subsomwong Phawinee;Doohan Dalla;Fauzia Kartika Afrida;Akada Junko;Matsumoto Takashi;Yee Than Than;Htet Kyaw;Waskito Langgeng Agung;Tuan Vo Phuoc;Uchida Tomohisa;Matsuhisa Takeshi;Yamaoka Yoshio;Subsomwong Phawinee
• 通讯作者: Subsomwong Phawinee

Enhancement effector vesicles derived from Staphylococcus aureus on virulence of Pseudomonas aeruginosa

金黄色葡萄球菌来源的效应囊泡对铜绿假单胞菌的毒力增强

• 发表时间: 2022
• 作者: Subsomwong Phawinee;Doohan Dalla;Fauzia Kartika Afrida;Akada Junko;Matsumoto Takashi;Yee Than Than;Htet Kyaw;Waskito Langgeng Agung;Tuan Vo Phuoc;Uchida Tomohisa;Matsuhisa Takeshi;Yamaoka Yoshio;Subsomwong Phawinee;Subsomwong Phawinee
• 通讯作者: Subsomwong Phawinee

Role of Staphylococcus aureus-derived extracellular vesicles in pathogenicity of Pseudomonas aeruginosa

金黄色葡萄球菌来源的细胞外囊泡在铜绿假单胞菌致病性中的作用

• 发表时间: 2022
• 作者: Subsomwong Phawinee;Doohan Dalla;Fauzia Kartika Afrida;Akada Junko;Matsumoto Takashi;Yee Than Than;Htet Kyaw;Waskito Langgeng Agung;Tuan Vo Phuoc;Uchida Tomohisa;Matsuhisa Takeshi;Yamaoka Yoshio;Subsomwong Phawinee;Subsomwong Phawinee;Subsomwong Phawinee
• 通讯作者: Subsomwong Phawinee