The regulatory mechanism of sympathetic nerves on eosinophil-associated micr o-inflammation in animals with IBS

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This research aims to investigate the relationship and potential mechanism between sympathetic nervous system (SNS) and micro-inflammation. In this year, I established the maternal separation (MS) model to mimic IBS. This model showed visceral hypersensitivity to colorectal distention, which is the main pathophysiology of IBS. I confirmed that there is micro-inflammation in colon of MS rats at adulthood by flowcytometry method and IHC. Besides, I researched the SNS activity. The ELISA result showed that NE concentration in serum increased in the MS rats compared with control. Neural activation markers are higher in MS model rats compared with control. Taken together, I confirmed that MS rats showed over activation of SNS. In addition, I applied the 6-OHDA to degenerate the SNS, the pharmacological data suggested that SNS played a role in immune cell alteration in colon. I also started to establish the genetic modulation approach to specifically modulation SNS activity.