Intramyocardial magnetic targeting of cardiac myocytes

干细胞来源的心肌细胞移植是一种很有前途的治疗方法。目前的数据表明，移植细胞的植入和存活很少，这表明需要提高移植效率以及移植细胞的短期和长期植入。为了解决移植效率低的问题，我们假设在心肌细胞替代疗法的小鼠模型中，有可能显著增加移植心肌细胞的植入。对保留在靶组织中的移植细胞进行精确和可靠的量化是强制性的，并将通过一种成熟的基于实时聚合酶链式反应的方法进行。纵向分析将通过移植的心肌细胞的荧光素酶成像进行。

Transplantation of stem cell derived cardiac myocytes is a promising therapeutic approach. Present data demonstrate a minor engraftment and survival of transplanted cells pointing to a need to improve the transplantation efficiency as well as the short- and long-term engraftment of transplanted cells. In order to address the issue of poor transplantation efficiency we postulate strategies that have the potential to substantially increase the engraftment of transplanted cardiac myocytes in a murine model of cardiac cell replacement therapy. A precise and reliable quantification of transplanted cells retained in the target tissue is mandatory and will be performed by a well-established real-time-PCR based approach. Longitudinal analysis will be performed by luciferase imaging of transplanted cardiomyocytes.