Treatment of Periodontitis using stem cell like epithelial cell rests of Malassez

Malassez上皮细胞残余物（ERM）是干细胞样细胞的简单来源。我们以前的工作已经成功地证明了一个ERM变体（ERM的分离克隆）可以通过抑制牙齿和牙槽骨之间的强直来维持健康的牙周间隙。本研究旨在使用申请人先前分离的克隆模型来修复丢失或损伤的牙周膜（PDL）组织，以恢复牙齿的形状和功能。第1阶段：我们从猪的PDL组织中获得了原代ERM，并分离了ERM的克隆。我们分离克隆1-10。使用ERM细胞标志物的免疫荧光染色进行表征。

Epithelial cell rests of Malassez (ERM) are an easy source of stem cell-like cells. Our previous work has successfully demonstrated that an ERM variant (isolated clone of ERM) can maintain healthy periodontal space by inhibiting ankylosis between the tooth and alveolar bone.This study aims to use the applicant's previously isolated clone model to repair the lost or injured periodontal ligament (PDL) tissue to restore tooth form and function.Phase-1: We obtained primary ERM from PDL tissues of porcine and isolated clones of ERM. We isolated clones 1-10. The characterization was done using immunofluorescence staining of ERM cell markers.