Automated high-content screening microscope

所要求的高内涵成像显微镜系统将能够自动采集和定量分析成像数据。到目前为止，海因里希海涅大学还没有公开的高内涵显微镜系统。虽然海因里希海涅大学的先进成像中心（CAi）配备了高分辨率和超分辨率共聚焦显微镜，可用于低通量工作，但在自动化高内容成像显微镜系统领域存在不足。为了通过统计手段验证定量生物表型，如细胞脂质储存量、自噬或线粒体动力学，需要一种自动显微镜系统。有针对性地购买具有高达60倍放大率的自动共聚焦系统，将允许检测和量化尺寸范围为0.5至1.5 μm的非常小的特征。结合所提供的分析软件以及目前在图像分割和数据分析方面的专业知识（AG Beller），将有可能实现大量的应用。将该系统纳入计算机辅助信息基础设施将保障该系统可供更多的用户使用，并确保适当的硬件维护和用户支持。

The requested high-content imaging microscope system will enable the automated acquisition and quantitative analysis of imaging data. So far, there is no publicly accessible high-content-microscope system available at the Heinrich Heine University. While the Center of Advanced Imaging (CAi) of the Heinrich Heine University is well equipped with high- and super-resolution confocal microscopes for lowthroughput work, there is a deficit in the area of automated high-content imaging microscopy systems.In order to validate quantitative biological phenotypes - such as cellular lipid storage amounts, autophagy, or mitochondrial dynamics - by statistical means, an automated microscope system is needed. The targeted purchase of an automated, confocal system with up to 60x magnifications will allow the detection and quantification of even very small features in the size range of 0.5 to1.5 μm. In combination with the analysis software provided, and the present expertise in terms of image segmentation and data analysis (AG Beller), a plethora of applications will be possible. Integration of the system into the CAi infrastructure will safeguard the availability of the system to a larger user base as well and ensure appropriate hardware maintenance and user support.