Automated High-Throughput, High-Content Confocal/Wide-Field Microscope System

自动化高通量、高内涵共焦/宽视场显微镜系统

• 批准号: 9274625
• 负责人: Mary West
• 金额: $ 102.88万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2018-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274625
• 关键词: Address; Area; Automation; Biology; Biomedical Research; Cancer Biology; Cells; Core Facility; Data; Devices; Ensure; Faculty; Funding; Housing; Image; Image Analysis; Individual; Light; Liquid substance; Microscope; Microscopy; Minor; Molecular; Morphology; Organelles; Population; Price; Reporting; Research; Resolution; Sampling; Spatial Distribution; Speed; Stem Cell Research; Stem cells; Surveys; System; Testing; Time; United States National Institutes of Health; Vendor; Visualization software; analytical method; design; experimental study; flexibility; fluorescence imaging; high throughput screening; image processing; instrument; live cell imaging; operation; reconstruction; screening

PROJECT SUMMARY / ABSTRACT This proposal is for the purchase the Perkin Elmer (PE) Opera Phenix Automated High-throughput, High- content Confocal/Wide-field Microscope for the QB3 Shared Stem Cell Facility/High-Throughput Screening Facility (SSCF/HTSF). High-content screening (HCS) encompasses a set of analytical methods using automated microscopy, multi-parameter image processing, and visualization tools to extract quantitative data from cell populations. HCS typically employs fluorescence imaging of samples in a high-throughput format and reports quantitatively on parameters such as spatial distribution of targets and individual cell and organelle morphology. This proposed instrument system will enable high-content screens and other multi-conditional experiments that demand confocal imaging as opposed to wide-field imaging for their proper analysis, thereby greatly advancing the research capabilities of our faculty. We present the research of 5 Major Users and 4 Minor Users whose primarily NIH-funded, biomedical research would be carried forward through use of this instrument. Research topics include basic biology, cancer biology, and stem cell research. On the UC Berkeley campus there is currently no other instrument of its kind, and Berkeley faculty conducting HCS research are currently unable to conduct many classes of screens because there is no current capacity for high throughput acquisition of confocal images, including for example on cellular samples in multiple multi- well plates. As a core facility instrument, the proposed automated confocal microscope is designed for flexibility and can address an array of imaging needs such as confocal, transmitted light, live-cell imaging, wide-field imaging, time-lapse, and Z-stacks for 3D reconstruction and analysis. This instrument has the speed and accuracy that many of today’s experiments demand. In the past four years, PI Dr. Mary West has engaged in demonstrations of different versions of automated confocal imagers from various vendors and compared their capabilities to potential user needs. The instruments tested included the General Electric InCell 6000, the Molecular Devices Ultra and most recently, the IXM Confocal, and the Perkin Elmer Operetta. The PE Opera Phenix uniquely meets the needs of the multiple users surveyed, excels in several key areas of its design such as resolution and speed, and offers the best price point for a complete state of the art, turn-key system. Housing Opera Phenix in the HTSF with its particular HTS liquid handling automation capabilities, and staff expertise in facilitating screening experiments and image analysis will ensure successful instrument installation, operation and management as well as maximum accessibility and use.

项目总结/摘要 该提案是为了购买Perkin Elmer（PE）Opera Phenix自动化高通量，高 content用于QB 3共享干细胞设施/高通量筛选的共聚焦/宽视场显微镜 设施（SSCF/HTSF）。高含量筛选（HCS）包括一套分析方法， 自动显微镜、多参数图像处理和可视化工具，以提取定量数据 从细胞群体中。HCS通常采用高通量格式的样品荧光成像， 定量报告参数，如目标和单个细胞和细胞器的空间分布 形态学该拟议的仪器系统将使高内容屏幕和其他多条件 实验需要共焦成像，而不是宽场成像，以进行适当的分析，从而 大大提高了我们教师的研究能力。我们目前的研究5个主要用户和4 次要用户，其主要由NIH资助的生物医学研究将通过使用此 仪器研究课题包括基础生物学、癌症生物学和干细胞研究。 在加州大学伯克利分校的校园里，目前还没有其他同类的乐器，伯克利分校的教师进行 HCS研究目前无法进行许多类别的筛选，因为目前没有能力 对于共焦图像的高通量采集，包括例如在多个多- 好板。作为一个核心设施仪器，建议的自动共焦显微镜的设计灵活性 并且可以满足一系列成像需求，例如共焦、透射光、活细胞成像、宽视场成像、 成像、时间推移和Z-堆栈，用于3D重建和分析。该仪器具有速度和 当今许多实验所要求的准确性。 在过去的四年里，PI玛丽韦斯特博士参与了不同版本的自动化 共焦成像仪从不同的供应商，并比较他们的能力，潜在的用户需求。的 测试的仪器包括通用电气InCell 6000，分子器件超，以及最近， IXM Confocal和Perkin Elmer Operetta。PE Opera Phenix独特地满足 多个用户进行了调查，在分辨率和速度等几个关键设计领域表现出色， 最好的价格点为一个完整的国家的最先进的交钥匙系统。在HTSF中安置Opera Phenix， 特别是HTS液体处理自动化能力，以及促进筛选实验的工作人员专业知识 图像分析将确保仪器的成功安装、运行和管理， 最大限度地利用和使用。