Analysis for activation mode of respiratory neurons in the brainstem silice preparation by multichannel optical recording method

1.全动物制剂。(1)用玻璃微电极记录大鼠上颈C_（1，2）段呼气和吸气神经元的电活动。(2)将荧光染料坚牢蓝（Fast Blue）和二脒基黄（Diamidino Yellow）分别微量注射到猫上颈呼吸神经元群和膈运动神经元池中，对脑干背侧和腹侧呼吸神经元群的神经元进行双重标记.脑切片制备物的存活期：发现从断头大鼠分离的脑切片制备物（厚度，250 - 300 μ m）的存活期<micrn>对于视觉皮层和海马至少为7小时。以局部电刺激诱发的场电位的再现性来估计脑干的损伤时间为5小时.从脑切片制备物中对神经元活动的光学记录。(1)电刺激RH-155（NK=3041）或NK-2761（0.05 - 0.1%）染色的脑片，在视皮层和海马区均产生吸收信号。(2)这些信号的幅度与刺激强度无关，并可被局麻药甲哌卡因可逆地抑制。这表明这些信号与电活动相当。在脑干区的脑片标本中，获得了幅度相对较小的电活动和视信号。

1. Whole animal preparations.(1) The expiratory neuronal activities as well as the imspiratory ones were recorded extracelluarly in the upper cervical C_<1,2> segments in rats using glass-microelectrodes.(2) The micro-injection of the fulouresecnt dyes, Fast Blue and Diamidino Yellow, into the upper cervical respiratory neuron groups and phrenic motoneuron pools respectively, doubdly labeled the mose of the neurons in the dorsal and ventral resporatory neuron groups in the brainstem of the cat.2. Survival period of the brain slice preparations.The survival period fo the slice preparations (thickness, 250 -300 <micrn>m) of the brain isolated from decapitated rats, was found to be at least 7 hrs for the visual cortex and hippocampus. and 5 hrs for the brain stem, when estimated by reproducibility of the field potentials evoked by local electrical stimulation.3. Oprical recording of the neuronal activities from the brain slice preparations.(1) In the visual cortex and hippocampus of the brain slice preparation stained with the dya, RH-155 (NK=3041) ot NK-2761 (0.05 - 0.1%), absorption signals were induced y elceetrical stimulation.(2) The amplitudes of these signals were fependent on the stimulus intersitiy and were reversibly inhibited by the treament with local anaesthetics, mepivacaine hydrochloride. This suggests that these signals are comparable to the electrical activities. In the slice preparations of rom the brainstem region,electrical activities as well as oprical signals with relatively smaller amplitudes were obtained.