Electrochemical Micro-immunosensors Using Monoclonal Antibodies

使用单克隆抗体的电化学微免疫传感器

基本信息

批准号：
61870104
负责人：
TANIGUCHI Isao
金额：
$ 3.78万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

The objectives of the present research project have been to develop highly functional and micro immunosensors, and also to study fundamental aspects on such sensors for practical use. In the present study, electropolymerized films have,at the first time, been applied successfully as functional materials to prepare electrochemical immunosensors as described below.Potentiometric human chorionic gonadotropin (hCG) sensor: when tyramine (=p-(2-aminoethy1)-phenol) is polymerized from an alkline alcohol solution at a low current density (e.g., 60muA/cm^2), a pore-free and fine-textured film with low conductivity was obtained. Using this polytyramine modified electrode ( =0.5, =1-5 mm), on which monoclonal anti-hCG (against hCG- -subunit from mouse ascites fluid) was immobilized, a potentiometric hCG hormone sensor was prepared, which responded selctively, rapidly (< 5 min. for 90% response) and sensitively (detection limit of 1 ng/ml). also, using an another monoclonal antibody immobilized e … More lectrode as a reference one, non-immunochemical response was canceled and down to 1 ng/ml of hCG in a human serum was again determined.Highly sensitive enzyme immunosensor for human immunogloblin G (IgG): A porous polytyramine film was formed on a Pt electrode at first from an acid solution and the surface was further covered with a polytyramine film from alkaline alcohol solution to give a polytyramine doubly coated electrode, on which anti-IgG was then immobilizes through the peptide linkage. By means of the competitive enzyme immunoassay technique, IgG was determined in the concentration range of ca. 10 pg/ml to 1 mg/ml. For the present electrode, a mixture of 0.5%Tween-20, a biological detergent, and 1% bovine serum albumin (BSA) was effective to diminish nonspecific responses.New diode-type biosensors: Polypyrrole or polyaniline thin film was covered on gold film electrodes with a small gap; this device behaved as a diode becarse the conductivity of the polymer film changed with the oxidation state of the film. This device was applied to develop new biosensors for a flow injection system. Less

本研究项目的目标是开发高功能性和微型免疫传感器，也旨在研究此类传感器的基本方面。在本研究中，电电膜首次被成功地作为功能材料应用，以制备如下所述的电化学免疫传感器。静脉内的人绒毛膜促性腺激素（HCG）传感器：当硫氨酸（= p-（2-氨基乙醇）polymential（p-phenol depline de Alkline docrymenth）时， 60mua/cm^2），获得了无孔和细纹理的薄膜，具有低电导率。使用这种多锥虫修饰的电极（= 0.5，= 1-5毫米），将单克隆抗HCG（反对小鼠腹水的hcg- subunit）固定在其上，固定了一个电位的HCG HCG HORSENE传感器，制备了一个快速，<5 min for 90％的响应（for 90％），并响应90％（mm forsim for 90％），并响应90％的响应（m m）。同样，使用另一种单克隆抗体固定的E…更多的讲座作为参考，非免疫化学反应被取消，并再次确定了人血清中HCG的1 ng/ml HCG。对人免疫蛋白G（IgG）的高度敏感酶免疫传感器：首先是从酸溶液的PT电极上形成多孔的聚乙胺膜，并从酒精溶液中进一步覆盖了表面，从酒精溶液中进一步覆盖了多型膜，以使多型芳基胺的双重涂层电子通过，然后通过pypeptecage链接了pypeptece链接。通过竞争性酶免疫测定技术，在Ca的浓度范围内确定IgG。 10 pg/ml至1 mg/ml。对于当前电极，生物确定的混合物和1％牛血清白蛋白（BSA）可有效减少非特异性响应。New二极管型生物传感器：多吡咯或多苯胺薄膜在带有小毛间的金膜上覆盖了多苯胺或多苯胺薄膜；该设备表现为二极管，因为聚合物膜的电导率随膜的氧化状态而变化。该设备用于开发用于流动系统的新生物传感器。较少的