Mass-Marking Method for Fish Eggs and Larvae

本项目拟建立鱼卵和鱼苗的批量标记方法。在前期研究的基础上，提出了用荧光物质溶液浸泡卵和幼虫的方法进行耳石标记。(1)茜素络合酮（ALC）、四环素（TC）和钙蛋白蛋白（CAL）的标记剂筛选和最佳处理条件分别呈现出明显的红粉色、黄色和黄绿色荧光环。ALC和TC的最佳处理条件为50 ~ 200 mg/l和200 ~ 300 mg/l，处理约1 d。CAL就没那么成功了。(2)标记保留率和标记处理效果：TC和ALC处理在处理后5个月~ 2年的饲养期内，对阿育鱼和红鲷的生存和生长没有不利影响，每条鱼都有清晰的荧光标记。(3)耳石标记的可用性该技术已被证实可用于约25个物种，包括海洋和淡水物种。1987年和1988年在大分县小湾进行了alc处理红鲷鱼的标记重捕实验。实验取得了成功，并在野外调查中证实了该方法的有效性。

This project was planned to establish a method of mass-marking fish eggs and larvae. Based on thd preliminary works on the ayu, Plecoglossus altivelis, otolith-tagging by immersion eggs and larvae in solution of fluorescent sudstances was nesly developed as follows.(1) Screening of marking agents and optimum treatment condition Alizarin complexone (ALC), tetracycline (TC) and calsein (CAL) presented a distinct scarlet-pink, yellow and yellowish hreen fluorescent ring, respectively. Optimum conditions for ALC and TC teatment was 50-200 mg/l and 200-300 mg/l for about 1 day. CAL was less successful.(2) Mark retention and effect of marking treatment TC and ALC treatment had no unfavourable effect on survival and growth of the ayu and the red sea bream Pagrus major reared for 5 months to 2 years after treatment, and clear fluorescent marks were confirmed in every fish treated.(3) Availability of otolith-tagging This technique was confirmed to be available for about 25 species including both marine and freshwater species. Mark-recapture experiment of ALC-treated red sea bream was conducted in a small cove Oita prefecture in 1987 and 1988. Experinents were successful and we confirmed the availability of this method in a field survey.