Development of mass production techniques of glass eels for aquaculture based on recent advancements in eel ecology, physiology and behavior

根据野生鳗鱼的生理生态和环境信息，对淡水鳗鱼的人工成熟方法和幼鳗的饲养技术进行了改进。转录组分析表明，ara、aqp0、aqp3样基因mRNA表达量在自然成熟鳗鱼中显著高于人工成熟鳗鱼，可作为人工成熟鳗鱼获得优质卵的标记。通过对鳗鲡仔鱼体内渗透和营养稳态的分析，在鳗鲡仔鱼孵化后第2天观察其摄食海水和肠道内NKCC2b/NCCb的表达情况，并在孵化后第5天观察PEPT1定位于肠上皮细胞的顶端区域。这些结果有助于进一步改善鳗鱼幼体的饲养条件。通过对海洋雪幼虫体氨基酸氮的稳定同位素分析，发现海洋雪是野生幼虫的食物，在此基础上开发了一种新的幼虫饲料。

Based on ecophysiological and environmental information on the wild eels, the methods of artificial maturation of the freshwater eel and the rearing techniques of larvae were improved. Transcriptome analysis revealed that mRNA expressions of ara, aqp0, aqp3-like genes were significantly higher in naturally maturing eels than artificially matured ones, which can be used as markers for obtaining high quality eggs in artificially matured eels. According to the analyses for osmotic and nutritional homeostasis in eel larvae, ingested seawater and intestinal NKCC2b/NCCb expressions were observed after 2 days post-hatching, and PEPT1 was localized on the apical region of intestinal epithelial cells after 5 days post-hatching. These results contribute to further improvement of rearing conditions for eel larvae. A new type of larval diet has been developed based on the marine snow that was found to be food for wild larvae by the stable isotopic analysis of amino acid nitrogen of larval body.