Development of mass production techniques of glass eels for aquaculture based on recent advancements in eel ecology, physiology and behavior

根据野生鳗鲡的生理生态和环境资料，改进了鳗鲡的人工成熟方法和苗种培育技术。转录组分析表明，ara、aqp 0、aqp 3-like基因在自然成熟鳗鱼中的表达量显著高于人工成熟鳗鱼，可作为人工成熟鳗鱼获得优质卵子的标记。根据渗透压和营养稳态分析，在仔鱼出壳后2 d观察到摄食海水和肠道NKCC 2b/NCCb的表达，在出壳后5 d PEPT 1定位于肠上皮细胞的顶端区域。这些结果有助于进一步改善鳗鱼仔鳗的饲养条件。通过对幼虫体氨基酸态氮的稳定同位素分析，发现海洋雪是野生幼虫的食物，并据此研制出一种新的幼虫饲料。

Based on ecophysiological and environmental information on the wild eels, the methods of artificial maturation of the freshwater eel and the rearing techniques of larvae were improved. Transcriptome analysis revealed that mRNA expressions of ara, aqp0, aqp3-like genes were significantly higher in naturally maturing eels than artificially matured ones, which can be used as markers for obtaining high quality eggs in artificially matured eels. According to the analyses for osmotic and nutritional homeostasis in eel larvae, ingested seawater and intestinal NKCC2b/NCCb expressions were observed after 2 days post-hatching, and PEPT1 was localized on the apical region of intestinal epithelial cells after 5 days post-hatching. These results contribute to further improvement of rearing conditions for eel larvae. A new type of larval diet has been developed based on the marine snow that was found to be food for wild larvae by the stable isotopic analysis of amino acid nitrogen of larval body.