Ultra-Fast Bio-Molecule Detection based on Radio-Frequency Nano-Pore and Nano-Channel Circuits

基于射频纳米孔和纳米通道电路的超快速生物分子检测

• 批准号: 411826126
• 负责人: Professor Dr. Robert H. Blick
• 金额: --
• 依托单位: Institut für Nanostruktur- und Festkörperphysik (INF)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/411826126?language=en
• 关键词: Ultra; Fast; Bio; Molecule; Detection

Nanopore (NP) based molecule detection is one of the emerging techniques in the field of applied bio-physics. However, due to the lack of radio-frequency (RF) measurement techniques single molecule characterization at high speed is still not established. Here, we will introduce a novel high-speed measurement technique to this field with GHz-bandwidth employing RF-reflectometry as the next generation readout mechanism. NPs already became a viable und highly functional platform for molecule detection and characterization for large bio-molecules. In our proposed work we want to expand the functionality of NP on-chip devices to the RF-range. We then want to combine our expertise in integrated electronic and micro-fluidic circuitry with nano-channel (NC) based molecule detection. This approach has several advantages in that it allows for ‘easy’ integration of nanoelectronic circuit elements in close proximity to the translocating DNA-strand. This is exactly what our RF-based readout electronics require, i.e. truly nanoscale electrodes (1 – 2nm width) for signal pick-up and some ‘real estate’ surrounding the signal zone in order to place other elements. As will be described in the proposal we are currently able to trace RF-signals for sub-micron sized pores, so that we can take our approach one step further by embedding a nanoelectronic RF-oscillator circuit in a NC setup. In addition this setup enables full optical access and a high degree of parallel processing.

基于纳米孔（NP）的分子检测是应用生物物理领域的新兴技术之一。然而，由于缺乏射频（RF）测量技术，单分子的高速表征仍然没有建立。在这里，我们将向该领域介绍一种新颖的高速测量技术，其具有 GHz 带宽，采用射频反射计作为下一代读出机制。纳米粒子已经成为一个可行且功能强大的平台，用于大生物分子的分子检测和表征。在我们提出的工作中，我们希望将 NP 片上器件的功能扩展到 RF 范围。然后，我们希望将我们在集成电子和微流体电路方面的专业知识与基于纳米通道 (NC) 的分子检测相结合。这种方法有几个优点，因为它可以“轻松”地将纳米电子电路元件集成到紧邻易位 DNA 链的位置。这正是我们基于射频的读出电子设备所需要的，即用于信号拾取的真正纳米级电极（1-2nm 宽度）以及信号区域周围的一些“不动产”，以便放置其他元件。正如提案中所述，我们目前能够追踪亚微米尺寸孔隙的射频信号，因此我们可以通过在数控装置中嵌入纳米电子射频振荡器电路来更进一步地采取我们的方法。此外，该设置还可以实现完全光学访问和高度并行处理。