Micro-Channel-Plates fabricated by 3D-Nanoprinting for protein mass spectrometry

用于蛋白质质谱分析的 3D 纳米打印微通道板

• 批准号: 469222030
• 负责人: Professor Dr. Robert H. Blick
• 金额: --
• 依托单位: Institut für Nanostruktur- und Festkörperphysik (INF)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/469222030?language=en
• 关键词: Micro; Channel; Plates; fabricated; 3D

One of the true technical marvels of the 20th century is the so-called micro-channel plate (MCP). This photon and particle detector, invented in 1957 by Russian physicists Khlebnikov and Melamid (Khlebnikov & Melamid, 1957), offers an amplification factor of 106 up to 107. Because of this tremendous gain MCPs have been used from early on in a wide range of detector applications. This includes low-level signal detection in particular important in astronomy and in electron microscopy, field emission for night vision goggles and for time-of-flight mass spectrometry, and any other application requiring incredible gain with high spatial and temporal resolution for imaging. While there have been a myriad of different configurations being described and patented over the last 60 years, the basic MCP features remained the same. That is going to change now with the advent of 3D-nanoprinting (3DN), which we intend to employ for fabricating novel MCP layouts with unprecedented imaging and enhanced amplification. The beauty of 3D-nanoprinted MCPs (in short 3DN-MCPs) is the adaptive quality, i.e. we will be able to redesign the complete structure and thus revolutionize the technique. Such a 3DN-MCP will impact a large number of scientific fields ranging from radiation and particle (i.e. electrons, neutrons, and proteins) detection over to mass spectrometry of proteins. In particular for detecting high-mass proteins relevant for virus and virus fragment detection such a novel 3DN-MCP is extremely useful. Hence, we deem the development of such a 3DN-MCPs as a novel device for mass spectrometry. If the outcome of our work is as successful as we believe it will be, we also foresee a potential industrial impact for high-throughput tracing of virus mutations.

20世纪世纪真正的技术奇迹之一是所谓的微通道板（MCP）。这种光子和粒子探测器由俄罗斯物理学家Khlebnikov和Melamid于1957年发明（Khlebnikov & Melamid，1957），提供了106到107的放大系数。由于这种巨大的增益，MCP很早就被广泛应用于探测器中。这包括在天文学和电子显微镜中特别重要的低电平信号检测，夜视镜和飞行时间质谱仪的场发射，以及任何其他需要高空间和时间分辨率成像的令人难以置信的增益的应用。虽然在过去的60年里有无数不同的配置被描述和专利，但基本的MCP功能保持不变。随着3D纳米打印（3DN）的出现，这将发生变化，我们打算采用3DN来制造具有前所未有的成像和增强放大的新型MCP布局。3D纳米打印MCP（简称3DN-MCP）的优点在于自适应性，即我们将能够重新设计完整的结构，从而彻底改变技术。这种3DN-MCP将影响大量的科学领域，从辐射和粒子（即电子，中子和蛋白质）检测到蛋白质的质谱分析。特别地，对于检测与病毒和病毒片段检测相关的高质量蛋白质，这种新型3DN-MCP是非常有用的。因此，我们认为这种3DN-MCP的发展是一种新型的质谱装置。如果我们的工作成果像我们相信的那样成功，我们还可以预见到高通量追踪病毒突变的潜在工业影响。