Dietary tuning of poison frog tadpole behavior and development

毒蛙蝌蚪行为和发育的饮食调整

• 批准号: 413437791
• 负责人: Dr. Eugenia Sanchez
• 金额: --
• 依托单位: Institut für Zoologie
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/413437791?language=en
• 关键词: Dietary; tuning; poison; frog; tadpole

Poison frogs are toxic due to alkaloids they are able to take up from the diet and bioaccumulate, and this toxicity correlates to their conspicuous coloration, known as aposematism. In a dendrobatid poison frog, Ranitomeya imitator, tadpoles are fed by their mothers, who periodically deposit alkaloid-containing unfertilized eggs for their consumption. Animal behaviors are coordinated by the central nervous system and influenced by early life diet. With the aim to investigate how feeding frequency and alkaloid consumption influence the development and behavior of poison frogs, I propose a project with different experiments: i) to conduct behavioral assays coupled to 3D immunohistochemistry (iDISCO) to identify differences in the neural circuitry for begging and aggressive behaviors in tadpoles under a control treatment, a fasting treatment (fed the same quantity but less frequently) and an alkaloid treatment (fed the same quantity and frequency but adding the alkaloid trans-Decahydroquinoline); ii) to evaluate how these treatments affect tadpoles’ metabolic rate and development, and if they have a carry-over effect on the juvenile metabolic rate and behavior; iii) to test if the intensity of these behaviors in juveniles is correlated to the expression of pertinent neuropeptides and receptors in the hypothalamus. This work will set a baseline to study the evolution of parental care in poison frogs and will pioneer in setting a neural and molecular basis to investigate the evolution of aposematism and aposematic behavior.

毒蛙由于生物碱而具有毒性，它们能够从饮食中占据并生物弥补，而这种毒性与它们的明显着色（称为末端）相关。在树枝状毒毒蛙中，ranitomeya模仿者，t剂量由母亲喂食，母亲会定期存放含生物碱的无育鸡蛋以供食用。动物行为由中枢神经系统协调，并受到早期饮食的影响。旨在调查喂食频率和生物碱消耗如何影响毒蛙的发展和行为，我提出了一个具有不同实验的项目：i）进行行为调查与3D免疫组织化学（IDISCO）耦合，以确定神经回路的差异，以确定在对照治疗的情况下，在对照治疗的情况下进行了相同的治疗（较差的art），但在对照方面进行了相同的治疗（又是一个相同的治疗方法），但又有一个相同的治疗方法（又是一个相同的治疗方法），但在对照（相同的）中（又是一个相同的治疗方法）数量和频率，但添加了生物碱反式核喹啉）； ii）评估这些治疗方法如何影响t的代谢率和发育，以及它们是否对少年的代谢率和行为产生了承担影响； iii）测试少年中这些行为的强度是否与下丘脑中相关神经肽和受体的表达相关。这项工作将设定一个基线来研究毒蛙中父母护理的演变，并将开拓神经元和分子基础，以研究授课和宣传行为的演变。