Regulation of long distance neuropeptide signaling in aging

衰老过程中长距离神经肽信号传导的调节

• 批准号: 413888359
• 负责人: Dr. Aygul Subkhangulova, Ph.D.
• 金额: --
• 依托单位: Institut für Biologie
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/413888359?language=en
• 关键词: Regulation; long; distance; neuropeptide; signaling

Biogenesis, transport and fusion of secretory vesicles play a fundamental role in cellular and organismal homeostasis. Vesicle trafficking in neurons faces particular challenges due to their highly polarized structure and long axonal projections. Impaired trafficking and release of neuronal vesicular cargos, such as classical neurotransmitters, neuropeptides, and neurotrophic factors, contribute to age-related diseases of the brain. In this project, I will examine contribution of the core components of the evolutionary conserved exocytotic machinery to the axonal trafficking and release of secretory granules in different neuronal types at different stages of their maturation. More specifically, I will use combination of genetic, electrophysiological and imaging tools to examine trafficking and release of neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) in mouse primary neurons from different brain areas (hypothalamus, hippocampus, cortex) and in mouse brain slices. Results of this study will promote our understanding of the age-related diseases of the central nervous system.

分泌囊泡的生物发生、运输和融合在细胞和生物体内平衡中起着重要作用。由于神经元的高度极化结构和长轴突投射，神经元中的囊泡运输面临特殊的挑战。神经元囊泡货物，如经典的神经递质，神经肽和神经营养因子的运输和释放受损，有助于与年龄相关的脑疾病。在这个项目中，我将研究的核心组成部分的进化保守的胞吐机制的轴突运输和释放的分泌颗粒在不同的神经元类型在其成熟的不同阶段。更具体地说，我将使用遗传学，电生理学和成像工具的组合来检查神经肽Y（NPY）和脑源性神经营养因子（BDNF）在小鼠不同脑区（下丘脑，海马，皮质）的原代神经元和小鼠脑切片中的运输和释放。本研究的结果将促进我们对中枢神经系统年龄相关疾病的认识。