权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

てんかんを併発する行動異常動物モデルを用いた免疫組織学的研究

使用行为异常癫痫动物模型进行免疫组织学研究

基本信息

批准号：
23929016
负责人：
飛鷹範明
金额：
$ 0.32万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Scientists
财政年份：
2011
资助国家：
日本
起止时间：
2011 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23929016/
关键词：
電撃けいれん(ECS)α4β2nAChR 免疫組織染色

项目摘要

【目的】てんかんに併発する精神障害の発症機序を明らかにするため,電撃けいれん(ECS)を反復負荷したラットの脳組織を用いて免疫組織学的検討を行った。Wistar系雄性ラットにECS(50mA,0.2sec)を7日間反復負荷することによりY-maze試験での自発的交替行動率の低下とOpen-field試験での自発運動量の増加が引き起こされる。我々は既に,これらの行動障害は抗てんかん薬の前投与によってけいれんを抑制することで改善されること,さらにアセチルコリンエステラーゼ阻害薬のphysostigmineならびにα4β2ニコチン性アセチルコリン受容体(nAChR)作動薬のABT-418が,自発的交替行動障害(短期記憶障害)を改善することを確認している。今回は,学習および記憶において重要な役割を担っている海馬および前頭前野にターゲットをあて,反復ECSに伴う行動障害に対するα4β2nAChRの関与について免疫組織学的研究を行った。【実験:免疫組織学的検討】実験にはWistar系雄性ラット(6-8週令)を用い,ECS(100V,50mA,0.2秒間,60Hz)を1日1回7日間反復負荷した。反復ECS負荷後24時間に脳灌流を行い,脳を取り出した。その後,作成した海馬および前頭前野の脳切片を作成し,AChRα4抗体を用いた酵素抗体法によって免疫組織学的染色を行った。【結果】α4nAChR陽性細胞数は,反復ECS負荷によって前頭前野の前辺縁領域および海馬のCA1とCA3領域でsham群と比較して有意に減少した。【考察】アセチルコリンエステラーゼ阻害薬のphysostigmineならびにα4p2nAChR作動薬のABT-418が,反復ECS負荷後による短期記憶障害を改善することを考え合わせると,反復ECS負荷による行動障害に前頭前野の前辺縁領域および海馬のCA1とCA3領域のα4β2nAChRが関連している可能性が示唆された。

[objective] to investigate the mechanism of mental disorders, to clarify the sequence of psychiatric disorders, to clarify the sequence of psychiatric disorders, and to use anti-tumor immunology (ECS) to evaluate the effectiveness of immunology. The alternating action rate of Wistar male turbot ECS (50mA recovery0.2sec) during 7 days was lower than that of the normal control group (50mA recovery0.2sec). The rate of alternation of Y-maze cycles was lower than that of Open-field cycles. We do not know if there is any damage to the health care system, but we do not know how to do it. We do not want to do anything to prevent the loss of health. We do not know if we are not responsible for the prevention and treatment of health problems. We do not know if we are not responsible for the prevention and treatment of health problems. We do not know whether to prevent the loss of health, we do not know whether to prevent the loss of health, we do not know whether to prevent the loss of health, we do not know how to prevent the disease, and we do not know whether to prevent the loss of health, we do not know how to prevent the disease, and we do not know whether to prevent the loss of health, we do not know if we are not responsible for the prevention and treatment of physostigmine, we do not know if we are going to have a problem with each other, we do not know if we are going to be able to Self-inflicted alternating action damage (short-term memory barrier) can improve the performance of the system and confirm the failure. This time, we are going to make a record of how important it is to carry out the study of anti-ECS activities in the front field of the sea, in response to the study of alpha 4 β-2nAChR and immunology in response to the disease. The male Wistar mice (6-8 cycles) were fed with ECS (100V 50mA 0.2s, 60Hz) for 1 day to 7 days. 24 hours after the anti-ECS load, the bank was perfused, and the output was collected. The AChR α 4 antibody was detected by enzyme antibody method and the immunological staining was performed. [results] the number of α-4nAChR sexual cells was significantly lower in the former field than in the previous field. [results] the number of α-4nAChR sexual cells was significantly lower in the former field than in the previous field. [results] the number of α-4nAChR sexual cells was significantly lower in the former field than in the previous field. [results] the number of cells in the field of α-sham was less than that in the field of CA3. [investigation] it is necessary to prevent the operation of physostigmine equipment, such as 4p2nAChR, to prevent short-term damage caused by ECS load, to improve short-term records, to improve health, and to prevent ECS payrolls. In the previous field, it is possible to instigate the possibility of causing damage in the field of sea, CA1 and CA3.