抗菌薬の抗菌効果へおよぼす局所麻酔薬の影響

局麻药对抗菌药物抗菌作用的影响

• 批准号: 08771895
• 负责人: 卯田 昭夫
• 金额: $ 0.64万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08771895/
• 关键词: リドカイン; 抗菌効果

抗菌薬の抗菌効果へおよぼす局所麻酔薬の影響つまり併用効果について検討する前に、まず歯科口腔外科領域で使用頻度の最も多い局所麻酔薬であるlidocaine単独の抗菌効果について以下の2項目について明らかにした。1、Lidocaineの最小発育阻止濃度局所麻酔薬の抗菌効果への期待は、感染症の治療薬としてよりも、術中および術直後の感染予防としての意味合いが強い。感染成立に必要な菌量はわずかであることから、低濃度の菌液に対するlidocaineの最小発育阻止濃度を測定した。被検菌はCation Supplemented Muller-Hinton broth pH7.2、(M-H broth)にて濃度は10から10^7colony forming unit(cfu)/mlの菌液とした。Lidocaineは原末を用い、M-H brothにて2.0%lidocaine溶液を作製、菌浮遊液とlidocaine溶液を混和(終末濃度10.0、5.0、2.5および1.2mg/ml)し培養後、菌の発育が認められない最小のlidocaine濃度をもって最小発育阻止濃度とした。10^2cfu/mlのStreptococciなどに対しlidocaineの1.2〜2.5mg/mlが抗菌効果を示した。2、口腔組織中lidocaine濃度lidocaineの局所麻酔下で外科処置を行った際の口腔組織中濃度の報告は無く、実際の臨床上どの程度の抗菌効果が期待出来るかは不明であることから、口腔組織中濃度の測定を測定した。8万分の1エピネフリン含有歯科用2%キシロカインカートリッジを用い、局所麻酔下にて外科処置を受けた患者を対象とした。得られた口腔組織はpH7.4のリン酸緩衝液を加えhomogenateを得、18時間cold extraction後上清を得て試料とした。Lidocaine濃度はFluorescence Polarization Immuno Assay法にて測定した。口腔組織中lidocaine濃度は歯肉:824.7±275.4(mean±SE(μg/ml))、骨:581.3±109.8、歯根膜:2109.6±958.1、歯髄:49.5±11.5、歯嚢:978.8±283.9であった。

The effects of antimicrobial agents on local anesthetic agents are discussed in the past and in the field of dental and oral surgery. The most frequently used local anesthetic agents are lidocaine alone. The following two items are listed. 1. The minimum development inhibition concentration of Lidocaine is the most important factor in the expectation of antibacterial effect, treatment of infectious diseases, prevention of infection during operation and after operation. To determine the minimum growth inhibition concentration of lidocaine for low concentrations of bacteria Cation Supplemented Muller-Hinton broth pH 7.2,(M-H broth) at a concentration of 10 − 10^7colony forming unit(cfu)/ml. Lidocaine was prepared from the original solution, M-H broth and 2.0% lidocaine solution, and the suspension solution was mixed with lidocaine solution (final concentration 10.0, 5.0, 2.5, and 1.2mg/ml). After incubation, the minimum concentration of lidocaine was determined for bacterial growth. Streptococci at 10^2 cfu/ml are effective against lidocaine at 1.2 ~ 2.5 mg/ml. 2. The concentration of lidocaine in oral tissues is unknown. The concentration of lidocaine in oral tissues is not reported during surgical treatment. The antibacterial effect of clinical level is expected. The concentration of lidocaine in oral tissues is determined. 80,000 patients were treated with oral medicine, 2% of them were treated with oral medicine, and the other 3% were treated with oral medicine. After obtaining the oral tissue, pH 7.4 and acid buffer solution were added to homogenate, and the supernatant was obtained after 18 hours of cold extraction. Lidocaine concentrations were determined by Fluorescence Polarization Immuno Assay. Lidocaine concentrations in oral tissues were 824.7±275.4(mean±SE(μg/ml)) in dental pulp, 581.3±109.8 in bone, 2109.6±958.1 in dental root membrane, 49.5±11.5 in dental pulp and 978.8±283.9 in dental pulp.

项目成果

卯田昭夫,加來洋子,佐久間俊,下坂典立,渋谷鉱: "Lidocaineの菌発育抑制効果" 日本麻酔・薬理学会誌. 19・2. 208-209 (1996)

Akio Uda、Yoko Kaku、Shun Sakuma、Noritsu Shimosaka、Mine Shibuya：“利多卡因对细菌生长的抑制作用”日本麻醉学和药理学会杂志 19・2（1996）。