The fundamental forces of ATP-driven membrane transport (A13#)

ATP 驱动的膜运输的基本力量 (A13

• 批准号: 417605373
• 金额: --
• 依托单位: Abteilung Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/417605373?language=en
• 关键词: fundamental; forces; ATP; driven; membrane

ATP-binding cassette (ABC) transporters are essential membrane proteins found in both eukaryotic and bacterial cells that facilitate the uphill transport of chemically diverse compounds. The molecular details of mechanochemical coupling of ATP-hydrolysis to conformational changes in those proteins remain to be elucidated. We here propose to study two ABC model systems with a combination of single-molecule fluorescence and force spectroscopy to characterize how the ATP-driven motor domain generates and transmits forces. With this we will unravel how chemical energy is converted into conformational changes of the pump domain that drive active transport.

ATP 结合盒 (ABC) 转运蛋白是真核细胞和细菌细胞中发现的重要膜蛋白，可促进化学多样性化合物的上坡转运。 ATP 水解与这些蛋白质构象变化的机械化学耦合的分子细节仍有待阐明。我们在这里建议研究两个结合单分子荧光和力光谱的 ABC 模型系统，以表征 ATP 驱动的运动域如何产生和传递力。通过这一点，我们将揭示化学能如何转化为驱动主动运输的泵域构象变化。