AvbitravilyPriwedPCR法によるヒトがんの分子的核型分析法の確立

AvbitravilyPriwed PCR方法建立人类癌症分子核型分析方法

• 批准号: 09770135
• 负责人: 安田 純
• 金额: $ 1.22万
• 依托单位: National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09770135/
• 关键词: 放射線照射ヒト齧歯類雑種細胞DNAパネル; AP-PCR法; SHARP法

放射線照射ヒト齧歯類雑種細胞DNAパネル(放射線照射雑種細胞パネル)によるヒトゲノムArbitrarily Primed Polymerase Chain Reaction(AP-PCR)DNA指紋産物の詳細で効率的なマッピング技術の開発の第一段階として、AP-PCRの反応時のプライマーの鋳型配列への会合の特異性について基礎的な研究を行った。AP-PCRの反応条件ではプライマーまたは鋳型がそれぞれループ構造をとり、会合の安定性を増している可能性があることを見いだし、論文にまとめ投稿した。また、ヒト腫瘍細胞で特異的な増幅を示したAP-PCR断片を放射線照射雑種細胞パネルを用いてマッピングし、近傍に存在するがん遺伝子をサザンブロットで解析し、遺伝子増幅を認めた。この解析の過程で放射線照射雑種細胞パネルによるヒトAP-PCRDNA指紋産物のマッピング技術に欠かせないSimultaneous Hybridization of Arbitrarily Primed PCR(SHARP)法を応用した。

Radiation exposure to several kinds of cell DNA equipment (radiation radiation for several cell types). Radiation exposure (AP-PCR) DNA refers to the first phase of the cycle cycle in which the frequency of radiation exposure (AP-PCR) is high. The first phase of the cycle, and the response time of the AP-PCR, will be in line with the research practice of the specific equipment base. AP-PCR reverses the condition, the condition, the situation, the possibility, the stability, the possibility, the contribution, the contribution. The frame of the cell sensor shows that the AP-PCR fragments are irradiated with radiation lines. The radiation of several kinds of cells is used. There is a device nearby, and there is an analysis of the radiation spectrum of the cell, and the frame of the cell is confirmed. In the process of radiation, there are several kinds of cell radiation. AP-PCRDNA means that the technology is not suitable for the use of the Simultaneous Hybridization of Arbitrarily Primed PCR (SHARP) method.

项目成果

Uematsu K, Yasuda J, Sekine T, Sugano K, Kudoh S and Sekiya T: "A new T-lymphocyte cloning assay for detection of in vivo mutations in the human hypoxanthine-guanine phosphoribosyltransferase gene." Environmental and Molecular Mutagenesis. 30. 31-39 (1997

Uematsu K、Yasuda J、Sekine T、Sugano K、Kudoh S 和 Sekiya T：“一种新的 T 淋巴细胞克隆测定，用于检测人次黄嘌呤鸟嘌呤磷酸核糖基转移酶基因的体内突变。”