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分子の共同作用に基づく分子認識系の創製と分離プロセスへの応用

基于分子协作的分子识别系统的构建及其在分离过程中的应用

基本信息

批准号：
11750668
负责人：
塩盛弘一郎
金额：
$ 1.41万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

ジ(2-エチルヘキシル)スルホコハク酸ナトリウム(AOT)が作る逆ミセルヘ種々のアルキルアミンを添加して水の抽出への影響を明らかにした.長鎖アミンを添加した系は,水相のpHを低下させると逆ミセルへの水の抽出が急激に低下し,逆ミセルが形成しなくなった.水の抽出が低下するpHは,長鎖アミンのpKaと対応しており,アンモニウムイオンの生成とアンモニウムイオンとAOTとのイオン対形成の反応平衡を考慮したモデルにより水のpH変化による抽出挙動を説明できた.これにより長鎖アルキルアミンを添加したAOT逆ミセル系の設計が可能となった.リゾチームをモデルタンパク質としてジオクチルアミン(DOA)とAOTを混合した逆ミセル系における正抽出および逆抽出挙動を詳細に検討した.特に逆抽出において,DOA,AOTおよびリゾチームの濃度を変化させて実験を行なった.タンパク質の逆ミセルからの逆抽出過程は,逆ミセルの破壊とタンパク質と直接相互作用しているAOTの除去の2段階で進行していることが示唆された.これらの結果に基づき,正抽出槽と逆抽出槽の間を有機相を循環させて連続抽出を行なった.正抽出速度に比べ逆抽出速度は極端に遅く,逆抽出槽において有機相を分散させて接触面積を増加させることにより連続抽出が可能であることを明らかにした.逆抽出条件での界面張力が大きく有機相を逆抽出槽に分散させても容易に相分離できることが明らかとなった.AOT逆ミセル抽出系において水溶性アミンやアミノ酸を水相に添加すると,pHにより水の抽出挙動が大きく変化した.pHを低下させると親水性の高い化合物の場合は水の抽出量は増加し,親水性の低い化合物の場合は水の抽出量は減少した.添加物質のpKaと対応して水の抽出が変化するpHが変わり,添加化合物とAOTとの静電相互作用により逆ミセルの構造が大きく変化することが分かった.

(2) there is a significant increase in the amount of water that can be extracted from the water supply system. (2) the AOT system is used to reverse the operation of several kinds of equipment. In the water phase, the pH is low, the water is low, the water is low, and the water is low. The pH of the water pump is low, the temperature is very low, and the temperature is very low. The water supply is very low, and there is no significant difference between the two. In this case, there is a negative balance caused by the extraction of the water supply, which is caused by the low temperature of the water supply. The effect of the pH on the extraction of the water supply has been verified. You may be aware of the possibility that the AOT inverse monitoring system settings may be affected. Do you want to know if you want to make sure that you have a problem? you need to know that there is a problem that there is a problem that you are in the process of reversing the operation. You need to know that you are in the process of reversing the operation. You need to know that you are in the process of reversing the operation. You are in the process of reversing the operation. In this paper, the reverse extraction method is used, and DOA,AOT is used to reduce the temperature. In order to reverse the extraction process, the direct interaction between the two segments of the AOT was removed to indicate that the extraction process was reversed. The results show that the extraction slot is in the opposite direction, and there is an opportunity to follow the monitoring link to pull out the line. The positive extraction speed is higher than the reverse extraction speed, and the reverse extraction slot has the opportunity to disperse the contact surface to increase the contact speed. Under the condition of reverse extraction, there is an opportunity to reverse the extraction tank, disperse, separate, separate, The extraction capacity of pH water is much higher than that of low pH water and high water-based compounds, while that of water-based low-content compounds is much lower than that of water-based low-content compounds. Add the compound "pKa", "pH", "static", "interaction", "inverse", "make", "chemical", "fractionation", and so on.