精神分裂病におけるオンコジーンc-fynの分子生物学的および遺伝学的研究

精神分裂症癌基因c-fyn的分子生物学和遗传学研究

• 批准号: 11770562
• 负责人: 大沼 徹
• 金额: $ 1.34万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11770562/
• 关键词: 精神分裂病; fyn; 前頭前野; 海馬; 分子生物学

平成12年度は、まず昨年度行ったc-fyn mRNAの定量を精神分裂病と健常対照群の前頭前野および海馬で行った。その結果、c-fyn mRNAは分裂病群の前頭前野Brodmann area9で有意に増加しており、その他の部位では有意差は認められなかった。さらに本年度は11年度と同様の対象群を用い、radioactive immunohistochemistryによるc-fynの免疫活性の比較定量を行った。c-fynの免疫活性の分布は、前頭前野では皮質の第II層からVI層にかけてほぼ均一に認められ、これは昨年度実験したmRNAの分布と一致した。興味深いことに海馬においては、mRNAの分布が豊富に認められたdentate gyrusに免疫活性はほとんど認められず、その他のCA4-1およびparahippocampal gyrusに認められた。この結果は、c-fynのmRNAは細胞分裂などが活発な顆粒細胞層に著しいが、その蛋白質発現は神経成長に関連する分子層に著しいという、c-fynのオンコジーンおよび神経成長因子としての特徴をよく反映していると思われた。さらにこれらfilmautoradiogramの結果を、画像解析機器Seescan(UK)を用いて免疫活性を定量し、それを分裂病群と対照群で比較したところ、前頭前野のBrodmann area10でfynの免疫活性が分裂病群で有意に増加していた。しかしながら、その他の前頭前野の部位(Brodmann area9、11)及び海馬では、分裂病群と対照群で有意差は認められなかった。本年度と昨年度の結果をまとめると、精神分裂病患者の前頭前野では、神経の成長や神経伝達に関連する物質であるc-fynの遺伝子および蛋白質発現に障害が生じている可能性が示唆された。

In the 12th year of Pingcheng, last year, we measured the number of patients with schizophrenia, c-fyn mRNA, schizophrenia, schizophrenia and schizophrenia. The results showed that Brodmann area9 in the anterior field of c-fyn mRNA mitotic disease group intended to increase the number of patients, and the location of the disease was intentionally affected. The immune activity of Elephant Elephant population (radioactive immunohistochemistry) and c-fyn (Elephant Elephant population) in the current year and November were quantified and compared. The distribution of c-fyn immunoreactivity was consistent with that of mRNA in the previous year. The distribution of immunoreactivity was consistent in the former field, the second, the second, the second and the last year. The distribution of mRNA was rich, rich, dentate gyrus, immunoreactive, CA4-1, parahippocampal gyrus, immunoreactive. The results showed that c-fyn mRNA cell mitosis, cell mitosis, cell cycle, protein protein showed that the growth and growth of the gene molecules were affected, and the growth factor of the cell, the growth factor, and the growth factor, were detected. The results of filmautoradiogram results, Seescan (UK) of portrait analysis machine were used to quantify the immune activity of mitosis, and the immunoactivity of mitotic disease groups were compared with each other. The immunoactive mitotic disease groups of the former field were Brodmann area10 immunoreactive. The parts of the front field (Brodmann area9, 11), the sea fish, the mitotic disease group, and the others were intentionally different from each other. The results of last year showed that patients with schizophrenia were suffering from diseases in the past, and that people with schizophrenia were suffering from diseases in the past. The ability to grow up and grow up is very important. The results of the year show that there is a significant difference in the possibility of impairing the health of c-fyn patients.