重度歯周炎罹患歯の残存歯根膜を根面に増殖させた歯牙の再植による歯周組織再生の研究

重度牙周炎牙根面残留牙周膜再植牙周组织再生研究

• 批准号: 11771354
• 负责人: 齋藤 彰
• 金额: $ 1.34万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11771354/
• 关键词: 歯根膜; 組織培養; 再植; 歯根膜培養; 歯牙移植; 歯周組織再生

重度歯周炎罹患歯の抜歯後,その歯を培地中に移しin vitroで残存歯根膜細胞を増殖し、歯根膜の欠如した部分を覆った後に移植を行い、広範囲にわたる結合性付着の獲得をねらう新しい歯周組織再生療法について、その可能性を検索する目的で以下の研究を行った。歯根膜が歯冠側4mmにわたって喪失した歯周炎罹患歯モデルを作成した後,in vitroで根尖側の残存歯根膜由来の増殖細胞が歯根全面を覆うまで培養を行う。その後、この歯を口腔内へ移植し,移植後4週における移植歯根面と周囲組織との間にどのような付着様式が生じるかを病理組織学的に検索した.その結果,上皮のdown growthはほとんど見られず,増殖細胞が被覆した根面と歯肉結合組織との間には結合組織性付着が生じ,残存歯根膜および増殖細胞が被覆した根面と歯槽骨との間には歯根膜様の組織が観察された.しかし移植歯根面の一部には根吸収も見られた。一方、歯周炎罹患歯モデルを作成後直ちに再植した場合,歯根膜を除去した部分は上皮のdown growthが生じ,結合組織性付着は残存歯根膜部分のみに見られ,新たな結合組織性付着の獲得は認められなかった.今回の研究の結果,たとえ重度歯周炎罹患歯であっても,in vitroで残存歯根膜細胞を増殖し、歯根膜の欠如した部分を覆うことで,移植歯根面に広範囲にわたる結合組織性付着の獲得が可能であることが明らかとなった。しかし今後、移植後に生じる根吸収について,その原因を明らかとし、根吸収が生じない方法として本方法を確立させる必要が生じた。

After suffering from severe periarthritis, the patient will have to move in The remaining root membrane cells in vitro are proliferated, the root membrane is partially covered and then transplanted, and the root membrane is combined Sexually transmitted diseases include new ones, peripheral tissue regeneration therapy, and possibilities. Purpose and research are as follows. After the root membrane is 4mm away from the coronal side and the root membrane is lost, Zhou Yan suffers from the disease and is made into The remaining root membrane on the apical side of the root is derived from the proliferating cells in vitro and the root is fully covered and cultured.その后、この歯をIntraoral transplantationし, 4 weeks after transplantation, transplantation of root surface and surrounding tissue between the teeth The result of pathological histology, epithelial down growthはほとんど见られず, the growth cells cover the root surface and the root surface combines with the flesh and the tissue combines with the tissue and the root membrane remains. The proliferating cells cover the root surface and the root surface and the root surface are absorbed. On the one hand, if you suffer from periodontitis and do not need to replant the root after it is made, the epithelial part of the root membrane will be removed and the epithelium will be down. growthが生じ, the combination of tissue-related attachments and the remaining part of the root membrane, 新たな combined with tissue-related attachments The result of the study is the result of the study, and the patient is suffering from severe inflammation of the week. The remaining root membrane cells in vitro are proliferated, and the root membrane is partially covered and transplanted歯根面に広风囲にわたるcombines the organizational pay to obtain がpossible であることが明らかとなった. In the future, after transplantation, there will be root absorption, root absorption, root absorption, and root absorption. This method is necessary to establish root absorption.