Isotope mass spectrometer (GC MS-IRMS-ASE)

土壤科学、有机地球化学和食品技术工作组联合申请了气相色谱-质谱仪-同位素质谱仪-加速溶剂萃取-偶联。该仪器将用于下列科学领域：(I)测定和量化植物材料、土壤和沉积物中的草药和微生物代谢物，以及测定所测代谢物的同位素比率；(Ii)将有机物质(13C、15N、D、18O)的特定位置稳定同位素标记与微生物生物标志物(PLFA、氨基糖)的特定成分同位素分析相结合，以分析和量化土壤和沉积物中单个微生物组的特定代谢途径，以及量化特定微生境中的元素周转；(Iii)该仪器将能够研究在食物的特殊储存条件下复杂体系中脂肪和蛋白质的共同氧化，并测定形成的氧化产物的同位素比率；(Iv)该仪器将通过追踪蓝藻在土壤和沉积物中的成岩转化产物，帮助确定最近输入到土壤和沉积物中的蓝藻。复杂介质(土壤、植物、沉积物、食品)中代谢物的组分特定稳定同位素分析是土壤-地球-生物科学之间的界面，需要克里斯蒂安-阿尔布雷希茨大学基尔的各个院系和研究所之间的跨学科方法和合作。共享的分析平台是这种机构间合作的先决条件。拟议的同位素质谱仪是这种分析平台的核心部分。

The working groups soil science, organic geochemistry and food technology jointly apply for a Gas Chromatograph-Mass Spectrometer-Isotope Mass Spectrometer-Accelerated Solvent Extraction-Coupling. The instrument will be used in the following scientific fields: (i) for the determination and quantification of herbal and microbial metabolites in plant material, in soil and in sediments, as well as for the determination of isotope ratios of the measured metabolites; (ii) to combine position-specific stable isotope labelling of organic substances (13C, 15N, D, 18O) with component-specific isotope analysis of microbial biomarkers (PLFA, amino sugar), in order to analyze and quantify specific metabolic pathways of individual microbial groups in soils and in sediments, as well as to quantify element turnover in specific microhabitats; (iii) the instrument will enable to study the co-oxidation of lipids and proteins in complex systems under characteristic storage conditions for food and the determination of isotope ratios of the formed oxidation products; (iv) and finally the instrument will contribute to the determination of the recent cyanobacterial input in soil and in sediments by tracing their diagenetic conversion products.Component specific stable isotope analysis of metabolites in complex media (soils, plants, sediments, food) represents an interface between soil-, geo- and biosciences and requires an interdisciplinary approach and cooperation between the individual faculties and institutes of the Christian-Albrechts-University Kiel. A shared analytics platform is a prerequisite for such an interinstitutional cooperation. The proposed isotope mass spectrometer is a central part of such an analytics platform.