Gatekeeper – Bioprinting in vitro mini-models with recapitulating in vivo anatomy of the blood-brain barrier using remodelable elastin-like protein bioinks and neural progenitor cells

Gatekeeper â 使用可重塑弹性蛋白样蛋白质生物墨水和神经祖细胞来生物打印体外微型模型，重现血脑屏障的体内解剖结构

• 批准号: 425869082
• 负责人: Professorin Dr.-Ing. Daniela Filipa Duarte Campos
• 金额: --
• 依托单位: Zentrum für Molekulare Biologie (ZMBH)
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/425869082?language=en
• 关键词: Gatekeeper; Bioprinting; vitro; mini; models

The blood-brain barrier (BBB) is a highly complex, multi-layered structure that serves as the gatekeeper between the central nervous system and the rest of the body. Three-dimensional BBB in vitro mini-models are potential platforms for understanding how the BBB functions, evaluating drug penetration across the barrier and investigating personalized therapies for neurodegenerative diseasesThe goal of Gatekeeper is to investigate the feasibility of using elastin-like protein (ELP) hydrogels with varying degradability degrees as bioinks for bioprinting in vitro mini-models with recapitulating anatomy of the BBB.The execution of Gatekeeper is divided in three parts: 1) development and characterization of ELP bioprintable bioinks, 2) printability testing and optimization of printing parameters of ELP bioinks and 3) bioprinting of BBB mini-models with primary murine neural progenitor cells.

血脑屏障（BBB）是一种高度复杂的多层结构，充当中枢神经系统和身体其他部分之间的守门人。三维血脑屏障体外微型模型是了解血脑屏障功能的潜在平台，评价药物穿过屏障的渗透性并研究神经退行性疾病的个性化治疗Gatekeeper的目标是研究使用具有不同降解程度的弹性蛋白样蛋白（ELP）水凝胶作为生物墨水用于体外微型生物打印的可行性。模型，概括了BBB的解剖结构。Gatekeeper的执行分为三个部分：1）ELP可生物打印的生物墨水的开发和表征，2）ELP生物墨水的印刷适性测试和印刷参数的优化，和3）用原代鼠神经祖细胞生物印刷BBB微型模型。