High-end mass spectrometer with nano-HPLC system

配备纳米高效液相色谱系统的高端质谱仪

• 批准号: 426546972
• 金额: --
• 依托单位: Justus-Liebig-Universität Gießen
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/426546972?language=en
• 关键词: end; mass; spectrometer; nano; HPLC

The comprehensive qualitative and quantitative analysis of complex proteomes and protein/protein interactions has become a central issue of many biomedical research projects today. Since posttranslational modifications (PTMs) play a central role in many biological processes the detection, localization and quantification of these modifications has become the central challenge in modern protein analysis. For the comprehensive analysis of common and rare PTMs high-end mass spectrometers are needed. Since many peptide modifications lead to a decreased ionization efficiency, sophisticated mass spectrometric methods are needed to detect these peptides in complex mixtures and to submit them to adequate fragmentation processes. The old MALDI-TOF-MS instrument purchased 15 years ago in our central protein analysis facility can no longer fulfill these requirements. Therefore, we apply for a new high-end ESI-MS instrument which completely fulfills all requirements. Besides high sensitivity, high mass resolution and mass accuracy the instrument allows many different fragmentation methods. For the central analytical core facility this instrument offers the best potential to perform all the envisaged projects.

复杂蛋白质组和蛋白质/蛋白质相互作用的综合定性和定量分析已成为当今许多生物医学研究项目的中心问题。由于翻译后修饰（PTMs）在许多生物学过程中起着核心作用，因此这些修饰的检测、定位和定量已成为现代蛋白质分析的核心挑战。为了全面分析常见和罕见的PTM，需要高端质谱仪。由于许多肽修饰导致电离效率降低，因此需要复杂的质谱方法来检测复杂混合物中的这些肽，并将其提交给适当的碎片化过程。15年前在我们的中心蛋白质分析设施中购买的旧MALDI-TOF-MS仪器不再能够满足这些要求。因此，我们申请了一种完全满足所有要求的新型高端ESI-MS仪器。除了高灵敏度、高质量分辨率和质量准确度外，该仪器还允许许多不同的裂解方法。就中央分析核心设施而言，这一仪器为执行所有设想的项目提供了最佳潜力。