Multiphoton laser scanning microscope for intravital imaging applications

本提案旨在建立一个多光子成像单元，用于活体成像应用。该装置将位于动物住房设施封闭的动物手术室内，这将使用户能够进行纵向重复调查，这些调查需要在不同的时间点将动物带进和带出住房设施。申请人专注于肿瘤，心血管和免疫学应用，明确侧重于先天和适应性免疫细胞的研究和跟踪。因此，将对心血管系统、心肌、原发性和继发性淋巴器官进行影像学检查。所申请的设置是为这些应用量身定制的，因为它允许跟踪组织中的细胞和快速活体成像。该显微镜将嵌入现有成像核心设施的结构中，并将提供高水平生命成像的可能性，并将填补现有成像平台内未满足的需求。

This proposal aims to establish a Multiphoton imaging unit for intravital Imaging applications. The set-up will be situated within the animal operation theatre enclosed in the animal housing facility, which will enable the users to perform longitudinal repetitive investigations that require the animals to be taken in and out of the housing facility at various time points. The applicants are focusing on oncological, cardiovascular and immunological applications, with a clear focus on the investigation and tracking of innate and adaptive immune cells. Therefore imaging of the cardiovascular system, myocardium, primary and secondary lymphoid organs will be performed. The set-up applied for is tailored for these applications in that it allows tracking of cells in tissue and fast intravital imaging. The microscope will be embedded within the structure of the existing imaging core-facilities and will provide the possibility for high-level intravital imaging and will fill an unmet need within the available imaging platforms.