Multiphoton Laser Scanning Microscope

多光子激光扫描显微镜

• 批准号: 10177356
• 负责人: M. Douglas Benson
• 金额: $ 82.91万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177356
• 关键词: Address; Area; Categories; Cells; Classification; Dental Enamel; Dentistry; Equipment; Faculty; Fee-for-Service Plans; Fibrillar Collagen; Fluorescent Probes; Funding; Grant; Image; Lasers; Microscope; Minerals; Neurobiology; Orofacial Pain; Pain; Palate Bone; Pathway interactions; Research; Research Infrastructure; Research Personnel; Research Project Grants; Sampling; Scanning; Site; Structure; Synapses; Technology; Texas; Tissues; Tooth structure; United States National Institutes of Health; Universities; bone; brain tract; cell motility; college; craniofacial development; falls; fluorescence imaging; instrument; multiphoton imaging; programs; second harmonic

PROJECT SUMMARY The Texas A&M University College of Dentistry maintains a vigorous and growing research program focused on three main areas: 1) mineralized tissue formation and destruction, 2) craniofacial development, and 3) neurobiology of pain. As the number and diversity of NIH-funded research projects has increased over the past several years, so has our need for research infrastructure. This application addresses a key point of that need with a request for funding to purchase a multiphoton (MP) laser scanning microscope. This instrument will allow us to image fluorescent probes deep within palate, bone, and tooth in a way that is impossible with our current equipment. It will be built around an inverted stage with an environmental chamber that will allow us to track the migration of cells in live tissue explants over long periods. Fibrillar collagens and enamel are the basis for mineralized tissue, and the unique ability of MP imaging to generate second harmonics in both will allow us view the intimate relationship of the bone and tooth matrices with the cells that produce them. The inclusion of multicolor confocal laser scanning capability on this MP scope means we will be able to observe multiple fluorescent cellular probes in cleared samples while simultaneously viewing the structure of their matrix niche with second harmonics. Our neurobiology faculty will use the deep imaging ability of MP technology to visualize brain tracts and synaptic connections in the pathways that transduce orofacial pain. Our investigators’ needs for this instrument fall under two main classifications. First are those who have been using expensive off-site MP instruments at fee-for-service facilities to advance the Specific Aims of their funded projects. Second are those whose funded projects would benefit greatly from MP imaging, but who have found it too impractical to incorporate without an instrument of our own. Both these categories are well represented in the Major User Group. Acquisition of this long overdue instrument will allow our already productive investigators better achieve the stated Aims of their grants and enable new advances that they could not otherwise achieve.

项目概要 德克萨斯 A&M 大学牙科学院保持着充满活力且不断发展的研究项目，重点关注 三个主要领域：1) 矿化组织的形成和破坏，2) 颅面发育，3) 疼痛的神经生物学。随着 NIH 资助的研究项目的数量和多样性在过去不断增加 几年来，我们对研究基础设施的需求也在增加。该应用程序解决了这一需求的关键点 请求资金购买多光子 (MP) 激光扫描显微镜。该仪器将 使我们能够以我们无法实现的方式对上颚、骨骼和牙齿深处的荧光探针进行成像 当前设备。它将围绕一个带有环境室的倒置舞台建造，使我们能够 长期跟踪活组织外植体中细胞的迁移。纤维状胶原蛋白和牙釉质是 矿化组织的基础，以及 MP 成像在两者中产生二次谐波的独特能力 让我们了解骨骼和牙齿基质与产生它们的细胞之间的密切关系。这 该 MP 示波器包含多色共焦激光扫描功能，这意味着我们将能够观察到 在透明样品中使用多个荧光细胞探针，同时观察其结构 具有二次谐波的矩阵利基。我们的神经生物学教师将利用 MP 的深度成像能力 该技术可将传递口面部疼痛的通路中的脑束和突触连接可视化。 我们的研究人员对该仪器的需求主要分为两类。首先是那些曾经 在收费服务设施中使用昂贵的场外 MP 工具来推进其资助的具体目标 项目。第二类是那些资助的项目将从 MP 成像中受益匪浅的人，但他们发现 如果没有我们自己的工具，合并是不切实际的。这两个类别都很好地体现在 主要用户组。获得这一迟来的工具将使我们已经富有成效的 研究人员更好地实现其资助的既定目标，并实现他们无法实现的新进展 否则达到。