MRI: Acquisition of a high speed multiphoton laser-scanning microscope for research and training at UNLV

MRI：购买高速多光子激光扫描显微镜，用于 UNLV 的研究和培训

• 批准号: 1726925
• 负责人: Laurel Raftery
• 金额: $ 99.86万
• 依托单位: University of Nevada Las Vegas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726925&HistoricalAwards=false
• 关键词: MRI; Acquisition; speed; multiphoton; laser

An award is made to the University of Nevada, Las Vegas (UNLV) to acquire a high-speed multiphoton laser scanning microscope system to view the behaviors of cells in living tissues with time-lapse images. UNLV is the only research-intensive university in southern Nevada, and the largest in the state. Expansion of the scope and impact of UNLV research activities is a high priority for the State of Nevada, and part of its plan to diversify the economy beyond the gaming and tourism industries. The UNLV Confocal and Biological Imaging Core provides training and support for laser-scanning fluorescence microscopy in a wide array of biological, environmental, and engineering applications. Graduate students from across UNLV and nearby post-graduate professional universities receive basic and advanced training in quantitative fluorescence microscopy techniques, advancing the national goal of developing a diverse STEM workforce. Graduate students use the majority of Core instrument time, and are assisted by collaborating undergraduate researchers. In additional, other undergraduate UNLV students will experience the new multiphoton technology through modules in advanced laboratory coursework. In 2015, UNLV was ranked as one of the 10-most diverse public universities in the US. UNLV is also a Minority-Serving Institution for Native American and Pacific Islander students and an emerging Hispanic Serving Institution. Tours and demonstrations will be provided to students from nearby high schools and two-year and four-year colleges through outreach activities. To further recruit a diverse range of students to STEM disciplines, videos showing the inner workings of plant and animal tissues will be developed and presented in Clark County K-12 classrooms. Similar presentations for Science Café Las Vegas will enhance scientific literacy by introducing the lay public to the fascinating behaviors of cells within living tissues. The newly acquired multiphoton laser scanning microscope provides advanced technology to obtain images from living tissues to depths of 1 mm. This instrument will be located in the UNLV Confocal and Biological Imaging Core, so that it will be available on a first-come, first-served basis to scientists and students across Nevada. Availability of this instrument eliminates the barrier of transporting samples to other states, and enables Nevada researchers to study living systems at new levels of sensitivity and depth. The high-speed imaging technology decreases tissue damage and increases sensitivity, allowing detection of fast-changing processes over periods of seconds to hours. This type of imaging is driving unexpected discoveries about the brain and how cells work together to build the architecture of many other organs. The advanced capabilities of multiphoton laser scanning microscopy will be used to study a wide range of biological and engineering systems, such as hormonal control of gland secretion, electrical oscillations in living mouse brain slices, repair and regeneration of eye tissues, organization of bio-fouling bacteria, and electrical activity of polymers for robotics and nanotechnology.

拉斯维加斯的内华达州大学（UNLV）获得了一项奖励，以获得一种高速多光子激光扫描显微镜系统，用于通过延时图像观察活组织中细胞的行为。UNLV是内华达州南部唯一的研究型大学，也是该州最大的大学。扩大UNLV研究活动的范围和影响是内华达州的高度优先事项，也是其计划的一部分，以实现经济多元化，超越游戏和旅游业。UNLV共聚焦和生物成像核心为激光扫描荧光显微镜在生物，环境和工程应用中的广泛应用提供培训和支持。来自UNLV和附近研究生专业大学的研究生接受定量荧光显微镜技术的基础和高级培训，推进发展多元化STEM劳动力的国家目标。研究生使用核心仪器的大部分时间，并由合作的本科研究人员协助。此外，其他本科UNLV学生将通过先进的实验室课程模块体验新的多光子技术。2015年，UNLV被评为美国10所最多样化的公立大学之一。UNLV也是一个少数民族服务机构，为美洲原住民和太平洋岛民的学生和新兴的西班牙裔服务机构。将通过外展活动为附近高中和两年制和四年制大学的学生提供图尔斯参观和示范。为了进一步招募各种各样的学生到STEM学科，展示植物和动物组织内部运作的视频将在克拉克县K-12教室中开发和展示。拉斯维加斯科学咖啡馆的类似演讲将通过向公众介绍活组织中细胞的迷人行为来提高科学素养。新获得的多光子激光扫描显微镜提供了先进的技术，以获得活组织的图像，深度为1毫米。该仪器将位于UNLV共聚焦和生物成像核心，使它将在先到先得的基础上提供给整个内华达州的科学家和学生。该仪器的可用性消除了将样品运输到其他州的障碍，并使内华达州的研究人员能够以新的灵敏度和深度研究生命系统。高速成像技术减少了组织损伤，提高了灵敏度，允许在几秒钟到几小时的时间内检测快速变化的过程。这种类型的成像正在推动有关大脑以及细胞如何共同构建许多其他器官结构的意想不到的发现。多光子激光扫描显微镜的先进功能将用于研究广泛的生物和工程系统，如腺体分泌的激素控制，活小鼠脑切片的电振荡，眼组织的修复和再生，生物污染细菌的组织，以及用于机器人和纳米技术的聚合物的电活性。