Modulation of the in situ pellicle by alkanna tinctoria roots in oleum oliva (olive oil)

龋齿和侵蚀的发生是基于牙齿表面的生物粘附过程。因此，牙膜作为这些生理和病理生理过程和牙表面的中介。可以通过应用生物或仿生制剂有意地修饰膜层，以改善保护性能，如对侵蚀的保护作用。此外，可以减少微生物的粘附。目的：本临床实验研究旨在探讨橄榄油脂质胶束作为一种可能的生物运输系统的应用，这种生物运输系统可以从山扁桃根中提取生物活性物质。此外，将在原位条件下评估橄榄油中黑矾根对口腔初始生物粘附的影响。因此，一个主要的焦点将是在橄榄油中应用黑矾根作为防止侵蚀的保护，改变膜的超微结构和可能减少初始细菌定植。方法：将牛牙釉质板口服（n= 6例）。在膜形成1分钟后，受试者用10ml的测试溶液（对照组不冲洗，带橄榄油的黑石根，不带橄榄油的黑石根）冲洗10分钟。之后，对每个测试溶液和每个受试者进行以下实验：淀粉酶、溶菌酶、过氧化物酶和GTF酶活性测定。此外，将进行荧光显微镜观察细菌粘附和钙/磷酸盐释放的光度测量。此外，通过透射电镜观察4名受试者的膜膜超微结构和HCL孵育后的膜膜超微结构中的脂质胶束。观点：这项研究将从根本上有助于理解口腔生物粘附过程的改变。此外，脂质胶束与白姜根有效物质的结合可以作为预防性牙科创新生物学方法发展的基础。

Introduction: The incidence of caries and erosion is based on bioadhesion processes at the tooth surface. Thereby, the pellicle serves as a mediator of these physiological and pathophysiological processes and the tooth surface. It is possible to modify intentionally the pellicle layer by the application of biological or biomimetic preparations in order to improve the protective properties, like the protective effect against erosion. Further, the adhesion of microorganisms can be reduced. Aims: The proposed clinical-experimental study aims to examine the application of lipid micelles from olive oil as a possible biological transport system for bioactive substances from Alkanna tinctoria roots. Furthermore, the effect of Alkanna tinctoria roots in olive oil on the initial oral bioadhesion will be evaluated under in situ conditions. Thereby, a main focus will be the applicability of Alkanna tinctoria roots in olive oil as a protection against erosion, the modification of the pellicle ultrastructure and the possible reduction of initial bacterial colonization.Methods: Bovine enamel slabs will be carried intraorally (n= 6 subjects). After 1 min of pellicle formation, the subjects rinse with 10 ml of the test solutions (control without rinsing, Alkanna tinctoria roots in olive oil, Alkanna tinctoria roots without olive oil) for 10 min. Afterwards, the following experiments will be carried out per test solution and per subject: Enzyme activity measurements of amylase, lysozyme, peroxidase and GTF. Furthermore, fluorescence microscopy to visualize bacterial adhesion and photometrical measurements of calcium/phosphate release will be performed. In addition, a visualization of lipid micelles in the pellicle ultrastructure and the pellicle ultrastructure after incubation with HCL will take place in 4 subjects with transmission electron microscopy.Perspective: The investigations will contribute fundamentally to the understanding of the modification of oral bioadhesion processes. Furthermore, the combination of lipid micelles and the potent substances from Alkanna tinctoria roots could serve as a basis for the development of innovative biological approaches in preventive dentistry.