Modulation of the in situ pellicle by alkanna tinctoria roots in oleum oliva (olive oil)

油橄榄（橄榄油）中的紫丁香根对原位表膜的调节

• 批准号: 426852015
• 负责人: Dr. Jasmin Kirsch
• 金额: --
• 依托单位: Professur für Kariologie, Zahnhartsubstanzlehre und Endodontie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/426852015?language=en
• 关键词: Modulation; situ; pellicle; alkanna; tinctoria

Introduction: The incidence of caries and erosion is based on bioadhesion processes at the tooth surface. Thereby, the pellicle serves as a mediator of these physiological and pathophysiological processes and the tooth surface. It is possible to modify intentionally the pellicle layer by the application of biological or biomimetic preparations in order to improve the protective properties, like the protective effect against erosion. Further, the adhesion of microorganisms can be reduced. Aims: The proposed clinical-experimental study aims to examine the application of lipid micelles from olive oil as a possible biological transport system for bioactive substances from Alkanna tinctoria roots. Furthermore, the effect of Alkanna tinctoria roots in olive oil on the initial oral bioadhesion will be evaluated under in situ conditions. Thereby, a main focus will be the applicability of Alkanna tinctoria roots in olive oil as a protection against erosion, the modification of the pellicle ultrastructure and the possible reduction of initial bacterial colonization.Methods: Bovine enamel slabs will be carried intraorally (n= 6 subjects). After 1 min of pellicle formation, the subjects rinse with 10 ml of the test solutions (control without rinsing, Alkanna tinctoria roots in olive oil, Alkanna tinctoria roots without olive oil) for 10 min. Afterwards, the following experiments will be carried out per test solution and per subject: Enzyme activity measurements of amylase, lysozyme, peroxidase and GTF. Furthermore, fluorescence microscopy to visualize bacterial adhesion and photometrical measurements of calcium/phosphate release will be performed. In addition, a visualization of lipid micelles in the pellicle ultrastructure and the pellicle ultrastructure after incubation with HCL will take place in 4 subjects with transmission electron microscopy.Perspective: The investigations will contribute fundamentally to the understanding of the modification of oral bioadhesion processes. Furthermore, the combination of lipid micelles and the potent substances from Alkanna tinctoria roots could serve as a basis for the development of innovative biological approaches in preventive dentistry.

简介：龋齿和侵蚀的发病率是基于牙齿表面的生物粘附过程。因此，表膜充当这些生理和病理生理过程和牙齿表面的介体。可以通过施加生物或仿生制剂来有意地修改表膜层，以便改善保护性能，如抗侵蚀的保护效果。此外，可以减少微生物的粘附。目的：拟议的临床实验研究的目的是检查应用的脂质胶束从橄榄油作为一个可能的生物运输系统的生物活性物质从Alkanna tinctoria根。此外，将在原位条件下评价橄榄油中的Alkanna tinctoria根对初始口腔生物粘附的影响。因此，一个主要的焦点将是适用性的Alkanna tinctoria根橄榄油作为保护免受侵蚀，修改的表膜超微结构和可能减少初始细菌colonization.Methods：牛牙釉质板将进行口内（n= 6个科目）。薄膜形成1分钟后，受试者用10 ml供试品溶液冲洗10分钟（对照品不冲洗，橄榄油中的紫草根，不含橄榄油的紫草根）。然后，将对每种供试品溶液和每名受试者进行以下实验：淀粉酶、溶菌酶、过氧化物酶和GTF的酶活性测量。此外，将进行荧光显微镜检查以观察细菌粘附和钙/磷酸盐释放的光度测量。此外，可视化的脂质胶束的薄膜超微结构和与HCL孵育后的薄膜超微结构将发生在4个科目与transmittance electron microscopy.Perspective：调查将从根本上有助于口腔生物粘附过程的修改的理解。此外，脂质胶束和来自Alkanna tinctoria根的有效物质的组合可以作为预防牙科中创新生物方法的发展的基础。