Effect of milk and milk components on the in-situ pellicle

龋齿和侵蚀的发生归因于牙齿表面的生物粘附过程。在这种情况下，薄膜被认为是牙齿硬组织上发生的生理和病理生理过程的关键介质。由于特定唾液成分的选择性吸附，在很短的时间内在口腔中的所有硬表面上形成薄膜。通过应用生物或仿生制剂对这种生理蛋白质和糖蛋白层进行有意修饰以改善保护性薄膜性质似乎是口腔预防中的一种创新的前瞻性方法。在这种背景下，有必要注意到，牛奶含有各种酪蛋白，由于它们的胶束组织，它们似乎类似于参与薄膜形成的某些组分。因此，天然的以及改性的酪蛋白的吸附似乎是一种可能的尝试，以提高龋齿和侵蚀保护潜力的原位薄膜。此外，酪蛋白胶束可以作为潜在的纳米载体的保护性成分在牙齿表面的应用。然而，酪蛋白与原位薄膜的相互作用迄今尚未被详细研究。因此，提出的临床实验研究的目的是检查不同类型的牛奶，牛奶成分和牛奶衍生物对原位薄膜的影响。重点将给予透射电子显微镜调查的超微结构的修改，以及吸附的酪蛋白的原位pellicle;酪蛋白积累将通过ELISA定量。此外，将通过基于荧光的方法确定选定冲洗液的初始细菌定植。这些调查将从根本上有助于理解口腔生物粘附过程的修改。此外，还将为预防牙科的创新生物方法的发展奠定基础。

The incidences of caries and erosion are attributed to bioadhesion processes at the tooth surface. In this context, the pellicle is regarded as the crucial mediator for the physiological and pathophysiological processes occuring on the dental hard tissues. Due to the selective adsorption of specific salivary components the pellicle is formed within a very short time on all hard surfaces in the oral cavity. The intentional modification of this physiological protein- and glycoprotein layer by the application of biological or biomimetic preparations in order to improve the protective pellicle properties appears to be an innovative prospective approach in oral prophylaxis. Against this background, it is necessary to note that milk contains various caseins which due to their micellar organization appear to resemble certain components involved in pellicle formation. Hence, the adsorption of native as well as modified caseins seems to be a possible attempt to improve the caries- and erosion protective potential of the in-situ pellicle. Furthermore, casein-micelles could serve as potential nanocarriers for the application of protective components at the tooth surface. However, the interaction of caseins with the in-situ pellicle has so far not been investigated in detail. Therefore, the proposed clinical-experimental study aims to examine the effect of different types of milk, milk components and milk derivatives on the In-situ pellicle. Emphasis will be given to transmission electron microscopic investigation of ultrastructural modifications as well as the adsorption of caseins to the in-situ pellicle; the casein-accumulation will be quantified by ELISA. Additionally, initial bacterial colonization will be determined for selected rinses by fluorescence-based methods. The investigations will contribute fundamentally to the understanding of the modification of oral bioadhesion processes. Furthermore, a basis for the development of innovative biological approaches in preventive dentistry will be created.