Spectral Confocal Laser Scanning Microscopy

本文提出的光谱共聚焦激光扫描显微镜(CLSM)将取代我们在2002年购买的CLSM系统。此外，它还将剥离2012年购买的另一套系统。最近，由于探测器有缺陷，我们不得不关闭旧仪器。因为，我们有一个非常严重的瓶颈在学院的CLSM和一些研究人员有巨大的问题来成像和测量他们的所有样本(特别是三个系的主要用户细胞生物学和植物生物化学，发育生物学和神经生物学)。现有的仪器非常灵活和灵敏，但目前的用户负荷如此之高，以至于即使是参与两个合作研究中心和一个DFG研究生院的主要用户也无法对其所有样品进行成像和测量。因此，作为替代采购，我们提出了CLSM系统，该系统可以补充现有的系统，并允许上述部门的各个研究小组进行所有必要的实验。此外，在指导之后，我们还希望允许其他研究小组的临时用户按需成像和测量他们的样品。因此，需要一种高度灵活的激光共聚焦显微镜系统，允许用户在不同波长对固定样品和活物质进行成像和测量。该仪器还应允许FRET和FRAP测量。由于有大量不同的标本和使用者，成像速度快、灵活性高、灵敏度高、低光毒性和用户友好的系统是重要的标准。

The here proposed spectral confocal laser scanning microscope (CLSM) shall replace our CLSM system, which was purchased in 2002. Moreover, it shall disburden another system purchased in 2012. Recently, we had to shut down the old instrument after a defect of the detector. Since, we have a very severe bottleneck in CLSM at the faculty and a number of researchers have enormous problems to image and measure all their samples (especially the main users of the three departments Cell Biology and Plant Biochemistry, Developmental Biology and Neurobiology). The available instrument is very flexible and sensitive, but the user load is currently so high that not even the main users, which are involved in two CRCs and a DFG-graduate school, are able to image and measure all their samples. Therefore, as replacement purchase, we propose a CLSM system, which can complement the existing system and additionally allows the various research groups of the above-mentioned departments to perform all their necessary experiments. Moreover, after instruction, we would also like to allow occasional users of other research group to image and measure their samples on demand. Therefore, a highly flexible CLSM system is required, which allows users to image and measure fixed samples and living material at different wavelengths. The instrument shall also allow FRET and FRAP measurements. Due to the large number of different specimen and user, important criteria are fast imaging speed, flexibility, high sensitivity, low phototoxicity and a user-friendly system.