Confocal Laser Scanning Microscope

共焦激光扫描显微镜

• 批准号: 527538521
• 金额: --
• 依托单位: Medizinische Hochschule Hannover (MHH)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/527538521?language=en
• 关键词: Confocal; Laser; Scanning; Microscope

Our group is new to the MHH; hence it is essential that we equip our lab with the equipment necessary to continue performing cutting-edge research. We use the model organism Drosophila melanogaster under normal, stressed, and pathological conditions to study the cellular and molecular mechanisms underlying human diseases such as muscular dystrophy and neuropathological disorders. The main aim of our research is to explain the cellular processes and signaling pathways that ensure the maintenance and homeostasis of the organism and thus prevent the development of diseases. A confocal laser scanning microscope (LSM) is essential to our research, used in generating up to 80% of our data. For this reason, and since the requested LSM will experience usage nearly all of the daily working hours by our group, it is necessary that we acquire an instrument dedicated for our use. In addition, this microscope will be beneficial to all other groups of the institute for tissue and cell imaging. First, our investigations using this microscope will uncover how stress initiates subcellular phase separation and how miRNAs are involved in a wide range of pathologies in humans. These findings will enable the development of miRNA-based therapeutics for age- and stress-related diseases. Second, a comprehensive analysis of the conserved signaling cascades that induce the formation of an adult stem cell niche and the mechanisms that regulate tissue morphology and differentiation will lead to a better understanding of the principles of organogenesis, which is central to stem cell biology and regenerative medicine. Furthermore, bioengineering of stem cell niches for in vivo engraftment, maturation, and maintenance of stem cells could provide the basis for successful manipulation of organoids and their therapeutic use. The LSM requested here is intended to increase the quality and efficiency of data production. The microscope must meet the requirements of a high-resolution and powerful confocal microscope and should give the applicant group access to the possibilities of faster multi-channel 3D imaging. Therefore, resolution, speed, versatility, flexibility, and ease of use represent paramount requirements. General requirements: the microscope must 1) be usable for both fixed and living tissue; 2) be in an upright orientation; 3) possess ample space below the lens, and this space must be usable in a flexible manner; 4) have both a motorized Z-drive and XY stage; 5) be able to detect at least four fluorophores (e.g., DAPI, GFP, dsRed, and Cy5) with high resolution and sensitivity and with little spectral cross-talk; and 6) achieve a resolution in XYZ directions of 120x120x350 nm, and for live cell imaging experiments, achieve a speed of at least 18 frames per second with an XY resolution of 140 nm or better. It is important that all the information required for the projects we are working on has been included in the offer.

我们的团队是MHH的新手;因此，我们必须为我们的实验室配备继续进行尖端研究所需的设备。我们使用的模式生物果蝇在正常的，强调的，和病理条件下，研究人类疾病，如肌营养不良症和神经病理性疾病的细胞和分子机制。我们研究的主要目的是解释细胞过程和信号通路，确保生物体的维持和稳态，从而防止疾病的发展。共聚焦激光扫描显微镜（LSM）对我们的研究至关重要，用于生成高达80%的数据。由于这个原因，并且由于所要求的LSM将在我们小组的几乎所有日常工作时间内使用，因此我们有必要获得专用于我们使用的仪器。此外，这种显微镜将有利于研究所的所有其他小组进行组织和细胞成像。首先，我们使用这种显微镜的研究将揭示压力如何启动亚细胞相分离以及miRNAs如何参与人类的广泛病理学。这些发现将使基于miRNA的治疗方法能够用于年龄和压力相关疾病。其次，对诱导成体干细胞龛形成的保守信号级联和调节组织形态和分化的机制进行全面分析，将有助于更好地理解器官发生的原理，这对干细胞生物学和再生医学至关重要。此外，用于干细胞的体内植入、成熟和维持的干细胞小生境的生物工程可以为成功操纵类器官及其治疗用途提供基础。此处要求的最小二乘均值旨在提高数据编制的质量和效率。该显微镜必须满足高分辨率和强大的共聚焦显微镜的要求，并应使申请组获得更快的多通道3D成像的可能性。因此，分辨率、速度、多功能性、灵活性和易用性是最重要的要求。一般要求：显微镜必须1）可用于固定和活组织; 2）处于直立取向; 3）在透镜下方具有足够的空间，并且该空间必须以灵活的方式使用; 4）具有电动Z驱动器和XY载物台; 5）能够检测至少四个荧光团（例如，DAPI、GFP、dsRed和Cy 5）具有高分辨率和灵敏度并且具有很少的光谱串扰;以及6）在XYZ方向上实现120 × 120 × 350 nm的分辨率，并且对于活细胞成像实验，实现至少18帧每秒的速度，XY分辨率为140 nm或更好。重要的是，我们正在进行的项目所需的所有信息都已包含在报价中。