Genetic mechanisms involved in the neurogenesis during the postembryonic development in Drosophila melanogaster.

果蝇胚胎后发育过程中涉及神经发生的遗传机制。

• 批准号: 01480030
• 负责人: TANIMURA Teiichi
• 金额: $ 3.01万
• 依托单位: Biological Laboratory, kyushu University (1990-1991)Fukuoka University (1989)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480030/
• 关键词: Drosophila; Neurogenesis; Neuroblast; Enhancer Trap

I have applied the enhancer trapping screen to identify genes involved in the neurogenesis during the larval and pupal stages in Drosophila. A single P element which contains a lacZ reporter gene was mobilized by supplying a genomic transpose and was randomly integrated into the genome. I have screened about 800 transformant lines carrying a single P-lacZ vector. Lines with a lacZ expression in a subset of neurons or in the neuroblast of the central nervous system were identified. These strains should provide a way to identify genes involved in the postembryonic neurogenesis and also used as specific cell makers to study neurogenesis.

我已经应用增强子捕获筛选，以确定在果蝇幼虫和蛹阶段参与神经发生的基因。通过提供基因组转座来动员含有lacZ报告基因的单个P元件，并将其随机整合到基因组中。我已经筛选了大约800个携带单个P-lacZ载体的转基因株系。鉴定了在神经元子集或中枢神经系统的成神经细胞中具有lacZ表达的线。这些菌株将为鉴定胚后神经发生相关基因提供一种方法，并可作为研究神经发生的特异性细胞标记物。

项目成果

Kimura,K.-I.: "Mutants with delayed cell-death of the ptilinal head muscles in Drosophila." Journal of Neurogenetics. (1992)

Kimura,K.-I.：“果蝇尾叶头部肌肉延迟细胞死亡的突变体。”

谷村禎一: "行動ー神経系への分子遺伝的アプロ-チ.久田光彦,岡島昭編『現代動物学の課題』7巻『行動』" 学会出版センタ-, (1990)

Teiichi Tanimura：“行为：神经系统的分子遗传学方法。Mitsuhiko Hisada，Akira Okajima（编辑）现代动物学问题，第 7 卷“行为””学会出版中心，（1990）

Kimura, K. -I. & Tanimura, T.: "Mutants with delayed cell-death of the ptilinal head muscles in Drosophila." Journal of Neurogenetics. (1992)

木村，K.-I。

Yoshikawa, S., Tanimura, T., Miyawaki, A., Nakamura, M., Yuzaki, Furuichi, T. & Mikoshiba, K.: "Molecular cloning and characterization of the inositol 1, 4, 5-triphosphate receptor in Drosophila melanogaster." journal of Biological Chemistry. (1992)

吉川，S.，谷村，T.，宫胁，A.，中村，M.，Yuzaki，Furuichi，T.

Kimura,K.ーI.: "Mutants with delayed cellーdeath of the ptilinal head muscles in Drosophila." Journal of Neurogenetics. (1992)

Kimura, K.-I.：“果蝇尾叶头部肌肉细胞死亡延迟的突变体。”神经遗传学杂志（1992）。