Molecular neurophysiology of neural mechanisms of taste reception in Drosophila

果蝇味觉感受神经机制的分子神经生理学

• 批准号: 17370027
• 负责人: TANIMURA Teiichi
• 金额: $ 9.34万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17370027/
• 关键词: Drosophila; taste; electrop hysiolo gy; receptor; projection pattern; G-protein

(1) We showed that a G-protein gamma subunit is partially required for sugar taste reception in Drosophila. The gene for the G-protein gamma subunit is expressed mainly in one of the gustatory receptor neurons. Behavioral and electrophysiological responses of the flies to sucrose were reduced by the targeted neural suppression of Gel gamma subunit-expressing cells utilizing Ga14/UAS system. We also demonstrated that responses to sugars were lowered in Gcl gamma null mutant.(2) Insects have specialized gustatory cells responding to water. We found a Ga14 enhancer trap strain in which GAL4 is expressed in a single taste receptor cell in each sensillum on the labellum. The neural function of these cells was blocked by expressing shibire [ts1] and behavioral tests and electrophysiological recordings indicated that they are the water taste cells. We show that axons of the water receptor cells project to a specific region in the subesophageal ganglion.(3) Sex pheromones are volatile or non-volatile in Drosophila. Volatile pheromones are sensed by olfactory neurons and we found that labellar Gr66a-expressing taste neurons respond to a male specific non-volatile pheromone, 7-tricosene. This was done by a newly developed tungsten electrode recording method. 7-Tricosene is probably sensed by tarsal taste sensilla during courtship and we observed projection patterns from Gr66a-expressing neurons in tarsi. By using a Flp-out technique, we generated mosaic flies in which only a few Gr66a-expressing neurons can be labeled and observed their projection patterns. Results showed that projection patterns of Gr66a-expressing neurons from sensilla that might sense the sex-pheromone and those from other sensilla that are involved in feeding behavior are different. We suggest that information from Gr66a-expressing neurons is sent to a different region depending on their related behavior.

(1)我们发现，一个G-蛋白γ亚基是部分所需的糖味觉接收果蝇。G蛋白γ亚基的基因主要在味觉受体神经元中表达。利用Ga 14/UAS系统靶向神经抑制表达Gel γ亚基的细胞，降低了果蝇对蔗糖的行为和电生理反应。我们还证明了在Gcl γ无效突变体中对糖的反应降低。(2)昆虫有专门的味觉细胞对水做出反应。我们发现了一种Ga 14增强子捕获菌株，其中GAL 4在唇瓣上的每个感器中的单个味觉受体细胞中表达。这些细胞的神经功能通过表达shibire [ts 1]而被阻断，行为测试和电生理记录表明它们是水味细胞。我们发现，水受体细胞的轴突项目在食管下神经节的一个特定区域。(3)果蝇的性信息素有挥发性和非挥发性两种。挥发性信息素的嗅觉神经元，我们发现，标签Gr 66 a表达的味觉神经元响应于男性特定的非挥发性信息素，7-tricopyrine。这是由一个新开发的钨电极记录方法。7-Tricobacterium可能是由跗骨味觉感受器在求偶过程中，我们观察到的投射模式，从Gr 66 a表达神经元在跗骨。通过使用Flp-out技术，我们产生了马赛克苍蝇，其中只有少数Gr 66 a表达神经元可以被标记，并观察它们的投影模式。结果表明，感受性信息素的感受器和其他参与摄食行为的感受器的Gr 66 a表达神经元的投射方式不同。我们认为，来自表达Gr 66 a的神经元的信息根据其相关行为被发送到不同的区域。

项目成果

Drosophila has two circadian clocks with different responsiveness to light

果蝇有两个生物钟，对光的反应不同

• 发表时间: 2005
• 期刊: European Journal of Neuroscience 22
• 作者: Yoshii;T.;Funada;Y.;et. al.
• 通讯作者: et. al.

Drosophila : a model system for taste

果蝇：味觉模型系统

• 作者: Teiichi;Tanimura
• 通讯作者: Tanimura

A inhibitory sex pheromone tastes bitter for Drosophila males.

对于雄性果蝇来说，抑制性性信息素的味道是苦的。

• 发表时间: 2007
• 期刊: PLoS ONE 2
• 作者: Lacaille;F.;Hiroi;M.;et. al.
• 通讯作者: et. al.

Central Projections of Tarsal Gustatory Receptor Neurons in Drosophila

果蝇跗骨味觉感受器神经元的中央投射

• 发表时间: 2007
• 作者: Teiichi Tanimura;Tsuyoshi Inoshita
• 通讯作者: Tsuyoshi Inoshita

Overlapping processing of sex pheromone and bitterness in Drosophila

果蝇性信息素与苦味的重叠加工

• 发表时间: 2006
• 作者: Fabien;Lacaille;Makoto;Hiroi;Robert;Twele;Tsuyoshi;Inoshita;Daisuke;Umemoto;Gerard;Maniere;Frederic;Marion-Poll;Mamiko;Ozaki;Wittko;Francke;Matthew;Cobb;Claude;Everaerts;Teiichi;Tanimura;Jean-Francois;Ferveur
• 通讯作者: Ferveur