Studies on structure and functions of silkworm proteins

蚕蛋白的结构与功能研究

• 批准号: 01480056
• 负责人: NAGATA Masao
• 金额: $ 4.54万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480056/
• 关键词: Silkworm; Proteins; 血液タンパク質

Insect haemolymph contained a large amount of proteins, which enormously varied with development. Many of these proteins have the same structure as a hexamer of subunits with about 80, 000 dalton, but their functions are different. There are two proteins belonging to a category of storage proteins in the silkworm, Bombyx mori. We investigated the structure and function of these storage proteins, arylphorin and larval female-specific protein. 1)Purification methods were improved to obtain a large amount of storage proteins. 2)Nature of storage proteins such as molecular weights of native proteins and subunits, isoelectric point, conjugated components were revealed. 3)Association, dissociation of subunits of storage proteins and agglutination of proteins under various conditions were analyzed. 4)Changes of protein metabolism and role of storage proteins during larval development were examined. 5)Relationship between storage protein concentrations and nutritional conditions of silkworm larvae was investigated.

昆虫血淋巴含有大量蛋白质，这些蛋白质随着发育而变化很大。许多这些蛋白质具有与约 80, 000 道尔顿的亚基六聚体相同的结构，但它们的功能不同。蚕（Bombyx mori）中有两种蛋白质属于储存蛋白类别。我们研究了这些储存蛋白、芳基蛋白和幼虫雌性特异性蛋白的结构和功能。 1)改进纯化方法，获得大量的储存蛋白。 2)揭示了储存蛋白的性质，如天然蛋白和亚基的分子量、等电点、缀合成分。 3)分析了不同条件下储存蛋白亚基的缔合、解离和蛋白的凝集情况。 4)研究了幼虫发育过程中蛋白质代谢的变化和储存蛋白的作用。 5)研究了家蚕幼虫贮藏蛋白浓度与营养状况的关系。

项目成果

Nagata, M. and Kobayashi, M.: "Quantitative changes in storage proteins during larval development of the silkworm, Bombyx mori." J. Seric. Sci. Jpn.59. 461-468 (1990)

Nagata, M. 和 Kobayashi, M.：“家蚕幼虫发育过程中储存蛋白的定量变化。”

Nagata, M. and Kobayashi, J.: "Effects of nutrition on storage proteins concentrations in the larval hemolymph of the silkworm, Bombyx mori." J. Seric. Sci. Jpn.59. 469-474 (1990)

Nagata, M. 和 Kobayashi, J.：“营养对家蚕幼虫血淋巴中储存蛋白浓度的影响。”

Nagata,M.: "Quantitative changes in storage proteins during larval development of the silkworm,<Bombyx>___ー <mori>___ー" 日本蚕糸学雑誌. 59. 461-468 (1990)

Nagata, M.：“蚕幼虫发育过程中储存蛋白的数量变化，<Bombyx>___<mori>___”《日本蚕丝学杂志》59. 461-468 (1990)。

Nagata,M.and Yoshikake,N.: "Protein metabolism in the forval development of the silkworm,Bombyx mori:Protein reutilization at the fourth molt." 日本蚕糸学雑誌. 58. 221-233 (1989)

Nagata, M. 和 Yoshikake, N.：“家蚕发育过程中的蛋白质代谢：第四次蜕皮时的蛋白质再利用”，58. 221-233 (1989)。

Nagata,M.: "Protein metabolism in the larval development of the silkworm ,Bombyx mori:Protein rentilization at the fourth molt" 日本蚕糸学雑誌. 58. 221-233 (1989)

Nagata, M.：“家蚕幼虫发育中的蛋白质代谢：第四次蜕皮时的蛋白质再生”，《日本丝绸学杂志》58. 221-233 (1989)。