Development of Methods for Insect Viruses

昆虫病毒方法的开发

• 批准号: 08306004
• 负责人: NAGATA Masao
• 金额: $ 5.63万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08306004/
• 关键词: Insect; Insect Viruses; Virus Infection

1. An atomic force microscope was applied to observation of insect viruses. Various methods for sample preparation were examined and an easy and rapid preparation of nuclear polyhedrosis virus was developed. 2. The proteins that appeared in the silkworm after infection of densonucleosis virus were analyzed and expression of them was revealed to be specific to the virus infection though they were uncoded to the virus genome. 3. Transcription products of densonucleosis were analyzed after developing a new method with recombination of their ORF and baculovirus vector. 4. Relationships between entomopox virus and parasitic insects in a host insect were examined and it was shown that virus infection affected on development of the parasitic insect. 5. An assay system of cell growth factors in the silkworm hemolymph that was based on in vitro cultivation of wing discs was established. 6. Genes of cytoplasmic polyhedrosis virus were expressed in the cultivated cells with using baculovirus vector system and specific antiserum to peptides coded in segment 8th and 9th was prepared for investigation of the virus replication.

1。将原子力显微镜用于观察昆虫病毒。研究了各种样品制备方法，并开发了核多面病毒病毒的简单快速制备。 2。分析了蚕病毒感染后出现的蛋白质，尽管未对病毒基因组进行未编码，但仍显示其表达对病毒感染具有特异性。 3。在开发了一种新方法的ORF和杆状病毒载体后，分析了致密性核细胞增多症的转录产物。 4。检查了宿主昆虫中昆虫毒素病毒与寄生虫昆虫之间的关系，并表明病毒感染受寄生虫发育的影响。 5。建立了基于机翼圆盘的体外培养的蚕血淋巴细胞生长因子的测定系统。 6。在培养细胞中使用使用杆状病毒载体系统表达细胞质多角质病毒的基因，并准备了在第8和9节中编码的肽的特异性抗血清，以研究病毒复制。

项目成果

Nakai, M.et al.: "Effects of the timing of entomopoxvirus administration to the smaller tea tortrix, Adoxophyes sp. (Lepidoptera : Tortricidae) on the survival of the endoparasitoid, Ascogaster retculatus (Hymenoptera : Braconidae)" Biol.Cont.13. 63-69 (1

Nakai, M.et al.：“昆虫痘病毒对较小的茶 tortrix Adoxophyes sp.（鳞翅目：Tortricidae）施用时间对内寄生蜂 Ascogaster retculatus（膜翅目：茧蜂科）存活的影响”Biol.Cont.13

Hagiwara, K., et al.: "Determination of the nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in BmN4 cells" J.Virol.72. 5862-5768 (1998)

Hagiwara, K., 等人：“家蚕细胞质多角体病毒片段 9 的核苷酸序列及其在 BmN4 细胞中的表达的测定”J.Virol.72。

Kawasaki,H.: "Transition from larvae to pupa:cell proliferation and gene expression in Bombyx mori." Invertebrate Reproduction and Development. 34. 101-108 (1998)

Kawasaki, H.：“从幼虫到蛹的转变：家蚕的细胞增殖和基因表达。”

Chen, S.Y.et al.: "A method for detecting growth factors of insects : assay system using mitosis and DNA synthesis in the wing disc cells of Bombyx mori" J.Ser.Sci., Jpn.67. 38-47 (1997)

Chen, S.Y.等人：“检测昆虫生长因子的方法：利用家蚕翅盘细胞有丝分裂和 DNA 合成的测定系统”J.Ser.Sci.，Jpn.67。

Sotoshiro, H.et al.: "Identification of a novel virus-specific in vitro translation product in the midgut of the silkworm, Bombyx mori, infected with B.mori densovirus type 2" J.Ser.Sci., Jpn.66. 38-47 (1997)

Sotoshiro, H.等人：“感染 2 型家蚕浓核病毒的家蚕中肠中新型病毒特异性体外翻译产物的鉴定”J.Ser.Sci.，Jpn.66。