Development of Methods for Insect Viruses

昆虫病毒方法的开发

• 批准号: 08306004
• 负责人: NAGATA Masao
• 金额: $ 5.63万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08306004/
• 关键词: Insect; Insect Viruses; Virus Infection

1. An atomic force microscope was applied to observation of insect viruses. Various methods for sample preparation were examined and an easy and rapid preparation of nuclear polyhedrosis virus was developed. 2. The proteins that appeared in the silkworm after infection of densonucleosis virus were analyzed and expression of them was revealed to be specific to the virus infection though they were uncoded to the virus genome. 3. Transcription products of densonucleosis were analyzed after developing a new method with recombination of their ORF and baculovirus vector. 4. Relationships between entomopox virus and parasitic insects in a host insect were examined and it was shown that virus infection affected on development of the parasitic insect. 5. An assay system of cell growth factors in the silkworm hemolymph that was based on in vitro cultivation of wing discs was established. 6. Genes of cytoplasmic polyhedrosis virus were expressed in the cultivated cells with using baculovirus vector system and specific antiserum to peptides coded in segment 8th and 9th was prepared for investigation of the virus replication.

1.将原子力显微镜应用于昆虫病毒的观察。本文对多种样品制备方法进行了研究，并建立了一种简便、快速的核型多角体病毒制备方法。2.对浓核病毒感染家蚕后出现的蛋白质进行了分析，发现这些蛋白质的表达是病毒感染所特有的，尽管它们是病毒基因组中未编码的。3.通过重组杆状病毒载体，建立了一种分析浓核病转录产物的新方法。4.研究了昆虫痘病毒与寄主昆虫的关系，表明病毒感染影响寄主昆虫的发育。5.建立了一种基于翅圆片体外培养的家蚕血淋巴细胞生长因子检测体系。6.利用杆状病毒载体系统在培养细胞中表达了胞质型多角体病毒基因，并制备了针对第8和第9节编码肽段的特异性抗血清，用于研究病毒的复制。

项目成果

Nakai, M.et al.: "Effects of the timing of entomopoxvirus administration to the smaller tea tortrix, Adoxophyes sp. (Lepidoptera : Tortricidae) on the survival of the endoparasitoid, Ascogaster retculatus (Hymenoptera : Braconidae)" Biol.Cont.13. 63-69 (1

Nakai, M.et al.：“昆虫痘病毒对较小的茶 tortrix Adoxophyes sp.（鳞翅目：Tortricidae）施用时间对内寄生蜂 Ascogaster retculatus（膜翅目：茧蜂科）存活的影响”Biol.Cont.13

Hagiwara, K., et al.: "Determination of the nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in BmN4 cells" J.Virol.72. 5862-5768 (1998)

Hagiwara, K., 等人：“家蚕细胞质多角体病毒片段 9 的核苷酸序列及其在 BmN4 细胞中的表达的测定”J.Virol.72。

Chen, S.Y.et al.: "A method for detecting growth factors of insects : assay system using mitosis and DNA synthesis in the wing disc cells of Bombyx mori" J.Ser.Sci., Jpn.67. 38-47 (1997)

Chen, S.Y.等人：“检测昆虫生长因子的方法：利用家蚕翅盘细胞有丝分裂和 DNA 合成的测定系统”J.Ser.Sci.，Jpn.67。

Kawasaki,H.: "Transition from larvae to pupa:cell proliferation and gene expression in Bombyx mori." Invertebrate Reproduction and Development. 34. 101-108 (1998)

Kawasaki, H.：“从幼虫到蛹的转变：家蚕的细胞增殖和基因表达。”

Sotoshiro, H.et al.: "Identification of a novel virus-specific in vitro translation product in the midgut of the silkworm, Bombyx mori, infected with B.mori densovirus type 2" J.Ser.Sci., Jpn.66. 38-47 (1997)

Sotoshiro, H.等人：“感染 2 型家蚕浓核病毒的家蚕中肠中新型病毒特异性体外翻译产物的鉴定”J.Ser.Sci.，Jpn.66。