Resistance of Insect to Pathogenic Viruses

昆虫对病原病毒的抗性

• 批准号: 10556012
• 负责人: NAGATA Masao
• 金额: $ 8.51万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10556012/
• 关键词: Insect; Defense system; 発育抵抗性; カイコ血液; アポトーシス

Nucleopolyhedrovirus (NPV) of the silkworm was inactivated after incubation with the silkworm hemolymph. Inactivation activity was located in the fraction that passed a filter with 5 kd M.W.and inhibitors of that activity existed in the fraction with more than 5Kd. Inactivation activity was retained after heat and protease treatment of the fraction. We also detected that glutathion inactivated NPV and are analyzing inactivation mechanism of NPV by the hemolymph and other compounds.An increase in median lethal dose (LD50) and median lethal time (LT50) values with increased larval age was recorded in bioassay studies of the nucleopolyhedrovirus (NPV) infected Spodoptera litura Fab. larvae. The LD50 values and LT50 increase with increased larval age. Larvae those were more than 11-day-old after hatching was not infected through oral inoculation even at the highest dose of the trial. However, intrahaemocoelic injection of NPV into matured larvae resulted into death due to viral infection indicating a lack of susceptibility possibly due to gut mediated barrier that developed as the larvae approached pupation.

家蚕核型多角体病毒（NPV）经家蚕血淋巴灭活后，其毒力明显下降。失活活性位于通过具有5kd M.W.的过滤器的级分中，并且该活性的抑制剂存在于大于5Kd的级分中。失活活性保留后，热和蛋白酶处理的馏分。通过对斜纹夜蛾（Spodoptera litura Fab）核型多角体病毒（NPV）的生物测定，发现随着幼虫龄期的增加，NPV的半数致死量（LD_（50））和半数致死时间（LT_（50））均增加。幼虫LD_（50）值和LT_（50）值随幼虫龄期的增加而增加。即使在试验的最高剂量下，经口接种孵化后11日龄以上的幼虫也未感染。然而，将NPV注入成熟幼虫的血体腔内导致死亡，这是由于病毒感染，表明缺乏易感性，可能是由于幼虫接近化蛹时形成的肠道介导的屏障。

项目成果

Monobrullah, MD.and Nagata, M.: "Immunity of lepidopteran insects against baculoviruses"J.ent.Res.. 23. 185-194 (1999)

Monobrullah, MD. 和 Nagata, M.：“鳞翅目昆虫对杆状病毒的免疫”J.ent.Res.. 23. 185-194 (1999)

Monobrullah,MD.and Nagata,M.: "Effects of larval age on susceptibility of Spodoptera litura (Lepidoptera : Noctuidae) to Spodoptera litura multiple nuclear polyhedrosis virus"Canadian Entomologist. 132. 337-340 (2000)

Monobrullah，MD.和Nagata，M.：“幼虫年龄对斜纹夜蛾（鳞翅目：夜蛾科）对斜纹夜蛾多核多角体病毒敏感性的影响”加拿大昆虫学家。

Katsuma,S. et al.: "Molecular characterization of baculovirus Bombyx mori nucleopolyhedrovirus polyhedron mutants"Archi.Virol.. 144. 1275-1285 (1999)

胜间，S.

Monobrullah,MD.and Nagata,M.: "Immunity of lepidopteran insects against baculoviruses"J.ent.Res. 23. 185-194 (1999)

Monobrullah，MD. 和 Nagata，M.：“鳞翅目昆虫对杆状病毒的免疫”J.ent.Res。

Monobrullah, MD.and Nagta, M.: "Effect of larval age on susceptibility of Spodoptera litura (Lepidoptera : Noctuidae) to Spodoptera litura multiple nuclear polyhedrosis virus"Canadian Entomologist. 132. 337-340 (2000)

Monobrullah, MD. 和 Nagta, M.：“幼虫年龄对斜纹夜蛾（鳞翅目：夜蛾科）对斜纹夜蛾多核多角体病毒敏感性的影响”加拿大昆虫学家。