Mechanism of infection and multiplication of the silkworm viruses

家蚕病毒的感染和繁殖机制

• 批准号: 07456033
• 负责人: NAGATA Masao
• 金额: $ 4.93万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456033/
• 关键词: Silkworm; Virus; Infection; Nuclear Polyhedrosis Virus; NPV

1.We detected differences in cytopathological changes with infection of BmNPV between two cultivated cell lines, BmN and l30 from the silkworm. The analyzes showed that differences existed in infectivity, multiplication rate and activity the virus and cell lethality between two cell lines. Apoptosis, which did not occur in BmN,was observed in l30 cells.2.A few cells survived in l30 cells after infection of high concentration of BmNPV.The cell lines cloned from them had various cytological and pathological characters, but their resistance to the virus was not high.3.Polyhedral protein synthesis did not correlate to particle replication of NPV,when amounts of plyhedral and virus particle proteins were measured under various conditions of infection with cell density and multiplicity of infection.4.Injury effects of the larval hemolymph on cultivated cells was observed and lipophorin was identified to be one of the injury factors.5.Ecdysteroid feeding to larvae at low concentrations had no effect on multiplication of NPV and cytoplasmic polyhedrosis virus.

1.我们检测到两种培养的细胞系，BMN和L30在蚕的细胞系中感染的细胞病理变化差异。分析表明，在感染性，繁殖率和活性中存在差异。两种细胞系之间的病毒和细胞致死性。 Apoptosis, which did not occur in BmN,was observed in l30 cells.2.A few cells survived in l30 cells after infection of high concentration of BmNPV.The cell lines cloned from them had various cytological and pathological characters, but their resistance to the virus was not high.3.Polyhedral protein synthesis did not correlate to particle replication of NPV,when amounts of plyhedral and virus particle proteins在各种感染条件下以细胞密度和感染的多重性进行测量。4。幼虫血淋巴对培养细胞的影响效应，并确定亲脂蛋白是损伤因素之一。5.Cecdysteloiloidfile file to arnpative for幼虫对NPV和细胞质质质质质体的繁殖无效。

项目成果

Abe, H., Obayashi, F., Harada, T., Shimada, T., Yokoyama, T.: "An application of DNA diagnosis method for preservation of the susceptible strain to densonucleosis virus type-1 of the silkworm, Bombyx mori" J.Seric.Sci.Jpn. 65 (3). 196-200 (1996)

Abe, H.、Obayashi, F.、Harada, T.、Shimada, T.、Yokoyama, T.：“DNA 诊断方法在保存家蚕浓核病毒 1 型易感菌株中的应用，家蚕

野口洋子,他: "ポリメラーゼ連鎖反応によるカイコ核多角体病ウイルスの検出技術の簡易化" 日本蚕系学雑誌. 64. 352-359 (1995)

Yoko Noguchi等人：“通过聚合酶链式反应简化蚕核多角体病毒检测技术”日本蚕学杂志64. 352-359 (1995)。

Naguchi, Y., Kobayashi, M.and Shimada, T: "An improved method for PCR-based detection of nuclear polyhedrosis virus in Bombyx mori" J.Seric.Sci.Jpn. 64 (4). 352-359 (1995)

Naguchi, Y.、Kobayashi, M. 和 Shimada, T：“一种基于 PCR 检测家蚕核多角体病毒的改进方法”J.Seric.Sci.Jpn。

阿部広明 et al: "カイコの系統保存へのDNA診断法の応用-濃核病ウイルスI型感受性系統の保存" 日本蚕糸学雑誌. 65(印刷中). (1996)

Hiroaki Abe 等人：“DNA 诊断方法在保存蚕品系中的应用 - 高核疾病病毒 I 型易感品系的保存”，日本血清学杂志 65（出版中）。

阿部広明,他: "カイコ濃核病ウイルス1型感受性系統保存へのDNA診断法の応用" 日本蚕糸学雑誌. 65. 192-200 (1996)

Hiroaki Abe 等人：“应用 DNA 诊断方法保存家蚕高核病毒 1 型易感株”，日本血清学杂志 65. 192-200 (1996)。