Mechanism of infection and multiplication of the silkworm viruses

家蚕病毒的感染和繁殖机制

• 批准号: 07456033
• 负责人: NAGATA Masao
• 金额: $ 4.93万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456033/
• 关键词: Silkworm; Virus; Infection; Nuclear Polyhedrosis Virus; NPV

1.We detected differences in cytopathological changes with infection of BmNPV between two cultivated cell lines, BmN and l30 from the silkworm. The analyzes showed that differences existed in infectivity, multiplication rate and activity the virus and cell lethality between two cell lines. Apoptosis, which did not occur in BmN,was observed in l30 cells.2.A few cells survived in l30 cells after infection of high concentration of BmNPV.The cell lines cloned from them had various cytological and pathological characters, but their resistance to the virus was not high.3.Polyhedral protein synthesis did not correlate to particle replication of NPV,when amounts of plyhedral and virus particle proteins were measured under various conditions of infection with cell density and multiplicity of infection.4.Injury effects of the larval hemolymph on cultivated cells was observed and lipophorin was identified to be one of the injury factors.5.Ecdysteroid feeding to larvae at low concentrations had no effect on multiplication of NPV and cytoplasmic polyhedrosis virus.

1.对家蚕细胞系BmN和130感染BmNPV后的细胞病理学变化进行了比较。结果表明，两种细胞系在病毒的感染性、增殖率、病毒活性和细胞杀伤力等方面存在差异。130细胞在感染高浓度BmNPV后仍有少量细胞存活，其细胞学和病理学特征多样，但对病毒的抵抗力不高。在不同的感染条件下，通过测定细胞密度和感染复数，观察了幼虫血淋巴对培养细胞的损伤作用，并鉴定了脂蛋白是其中的一种损伤因子低浓度蜕皮激素对NPV和胞质型多角体病毒的增殖没有影响。

项目成果

Abe, H., Obayashi, F., Harada, T., Shimada, T., Yokoyama, T.: "An application of DNA diagnosis method for preservation of the susceptible strain to densonucleosis virus type-1 of the silkworm, Bombyx mori" J.Seric.Sci.Jpn. 65 (3). 196-200 (1996)

Abe, H.、Obayashi, F.、Harada, T.、Shimada, T.、Yokoyama, T.：“DNA 诊断方法在保存家蚕浓核病毒 1 型易感菌株中的应用，家蚕

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Yoko Noguchi等人：“通过聚合酶链式反应简化蚕核多角体病毒检测技术”日本蚕学杂志64. 352-359 (1995)。

Naguchi, Y., Kobayashi, M.and Shimada, T: "An improved method for PCR-based detection of nuclear polyhedrosis virus in Bombyx mori" J.Seric.Sci.Jpn. 64 (4). 352-359 (1995)

Naguchi, Y.、Kobayashi, M. 和 Shimada, T：“一种基于 PCR 检测家蚕核多角体病毒的改进方法”J.Seric.Sci.Jpn。

阿部広明 et al: "カイコの系統保存へのDNA診断法の応用-濃核病ウイルスI型感受性系統の保存" 日本蚕糸学雑誌. 65(印刷中). (1996)

Hiroaki Abe 等人：“DNA 诊断方法在保存蚕品系中的应用 - 高核疾病病毒 I 型易感品系的保存”，日本血清学杂志 65（出版中）。

川崎秀樹,他: "カイコ終齢食桑期の翅原基における蛹化能力の獲得" 日本蚕糸学雑誌. 64. 487-492 (1995)

Hideki Kawasaki 等：“蚕最后摄食阶段翅膀原基的化蛹能力”，日本蚕丝学杂志 64. 487-492 (1995)。