Postnatal Development of Macromolecular Transmission in Intestinal Epithelium

肠上皮大分子传递的出生后发育

• 批准号: 01480100
• 负责人: HARADA Etsumori
• 金额: $ 4.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480100/
• 关键词: Intestinal Function; Macromolecular Transmission; Neonate; Colostrum; Postnatal Development; Gut Closure; Disaccharidases; Immunoglobulins; 初乳含有生理活性物質; 消化管ホルモン; 小腸上皮細胞; ガットクロ-ジャ-

The present experiments were carried out to clarify the developmental mechanism of gastrointestinal function using morphological and biochemical techniques in the suckling rats..1) Epidermal growth factor and insulin treatments caused the precocious cessation of intestinal macromolecular transmission and development of disaccharidase activeities. It is suggesting that in addition to corticosterone and thyroxine, insulin and EGF also play a role in the maturation of intestinal function.2) The role of endogenous CCK in the regulation of intestinal cessation and mucosal disaccharidase activities have been studied in the suckling rats. Oral administration of trypsin inhibitor (TI) induced precocious maturation of the small intestine which was not affected by CCK recrptor blocker. Thus, it is possible that precocious alteration in the intestine is attributable to the direct or indirect action of unknown factors derived from milk produced by TI.3) Secretin administrated subcutaneously or end … More ogenously released by oral administration of TI or hydrochloric acid solutions caused precocious changes in the intestinal function. Pentagastrin did not cause any changes in the intestinal development. These results suggest that secretin plays a role in the precocious maturation of the small intestine.4) Some crude fractions derived from bovine colostrum caused significant decline of macromolecular absorption from the intestine in the suckling rats.5) To investigate cellular mechanism of macromolecular absorption from intestinal epithelial cells, it was tried to improve the preparations such as reversed or non-reversed sac methods and Ussing typed chamber. A trace amounts of macromolecular marker, IgG, BSA or HRP was detected by ELISA methods in these preparations. However, further improvements of preparations were needed to analyze a characteristics of macromolecular absorption from epithelium in several kinds of animals and actions of biologically active substances against the developmental process of gastrointestinal tract in the newborn animals. Less

本实验利用形态和生化技术，对乳鼠胃肠功能发育机制进行了研究：1)表皮生长因子和胰岛素治疗可导致早熟大鼠肠道大分子转运中断和二糖酶活性发育。提示除了皮质酮和甲状腺素外，胰岛素和EGF在肠道功能成熟中也起作用。2)研究了内源性CCK在乳鼠肠道停搏和粘膜二糖酶活性调节中的作用。口服胰酶抑制剂(TI)可诱导小肠性早熟，CCK受体阻滞剂对此无影响。因此，肠道的早熟改变可能归因于未知因素的直接或间接作用，这些未知因素来自于皮下给药或End…给药所产生的乳汁。口服TI或盐酸溶液释放的更多基因会导致肠道功能的早熟变化。五肽胃泌素对肠道发育没有任何影响。这些结果提示促胰液素在小肠性早熟过程中起一定作用。4)牛初乳部分粗提物导致乳鼠肠道大分子吸收明显下降。5)为了探讨肠道上皮细胞大分子吸收的细胞机制，对制备方法进行了改进，如反向或非反向囊法和Ussing型小室。用ELISA法在这些制剂中检测到微量的大分子标志物--免疫球蛋白、牛血清白蛋白或辣根过氧化物酶。然而，为了分析几种动物上皮大分子吸收的特点以及生物活性物质对新生动物胃肠道发育过程的作用，制剂还需要进一步的改进。较少

项目成果

E.HARADA,Y.HASHIMOTO & B.SYUTO: "Epidermal Growth Factor Accelerates the Intstinal Cessation of Macromolecular Transmission in Suckling rat." Comparative Biochemistry & Physiology. 97A. 201-204 (1990)

原田荣、桥本勇

E.HARADA Y.HASHIMOTO & B.SYUTO: "Proceedings:XXIV WORLD VETERINARY CONGRESS" Gut Peptides Related with Precocious Maturation of Intestinal Function in Suckling Rats., (1991)

原田英和桥本

Etsumori HARADA & Bunei SYUTO: "Precocoious Maturation of Intestinal Function Induced by Oral Administration of Trypsin Inhibitor in Suckling Rats." Japanese Journal of Physiology. (1990)

原田悦守

H.KIRIYA,E.HARADA & B.SYUTO: "Analysis of Colostral Proteins in Calf Serum by Enzyme Linked Immunosorbert Assay." Journal of Dairy Science. 72. 398-406 (1989)

H.桐谷,E.原田

E. HARADA, Y. HASHIMOTO & B. SYUTO: "Synthetic Trypsin Inhibitor Influences Macromolecular Absorption from Small Intestine in the Sukling Rats."

E.原田，Y.桥本