The physiological role of blood-brain barrier and intestinal barrier during development of brain in the neonates.

血脑屏障和肠屏障在新生儿大脑发育过程中的生理作用。

• 批准号: 07456135
• 负责人: HARADA Etsumori
• 金额: $ 5.06万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456135/
• 关键词: Intestinal barrier; Blood-brain barrier; Biologically active substances in milk; Cerebrospinal fluid; Catecholamines in brain; Electric activities in brain; Entero-hepatic circulation; Docosahexaenoic acid; 脳機能の生後発達; 血液・脳関門; 初乳; 人口哺育; 生理活性物質 /

On the basis of characteristic transport of macromolecules from intestine and regulatory system of intestinal barrier to milk substances in the neonates, this investigation was curried out to examine the role of biologically active substances contained in milk to brain development in newborn animals.1. In order to evaluate the possibility of modification of brain function by colostral suckling, the transfer of colostral components into serum and cerebrospinal fluid has been studies by SDS electrophoresis, Western blotting and ELISA methods in nonsuckling neonatal pigs. Two colostral components (mwt.19,000 and 31,000) can be transported into cerebrospinal fluid via serum and one of them was identified beta-lactoglobulin. Thus, it was suggested that a possibility of modification of immatured brain functions by colostrum in neonatal pigs.2. Docosahexaenoic acid (DHA) is incorporated in large amounts in structural lipids of the developing central nervous systems. It was investigated the effects of DHA in the maternal diet on catecholamine contents in the brain and EEG in rat pups during the postnatal development. The results were suggested that the accumulated DHA in the brain has some effect on the activities of enzymes catalyzing the biosynthesis of catecholamine and on the neuronal excitation in the neonatal rat brain during the suckling period.3. Using the neonatal and weaning piglets, it was investigated the transfer of lactoferrin into bile via blood circulation from intestinal lumen. The results indicated that lactoferrin containning in milk is transported into blood circulation from intestine and excreted into bile, suggesting the possibility for entero-hepatic circulation of lactoferrin in neonatal pigs.

本研究根据新生动物肠道大分子转运的特点和肠道屏障对乳物质的调节系统，探讨乳中生物活性物质对新生动物脑发育的作用.为了探讨初乳哺乳对脑功能的影响，本实验采用SDS电泳、Western印迹和ELISA方法，研究了初乳成分向非哺乳仔猪血清和脑脊液中的转移。两种初乳成分（分子量19，000和31，000）可通过血清转运到脑脊液中，其中一种被鉴定为β-乳球蛋白。提示初乳对新生猪未成熟脑功能有一定的改善作用.二十二碳六烯酸（DHA）大量掺入发育中的中枢神经系统的结构脂质中。研究了母食中添加DHA对仔鼠脑组织中儿茶酚胺含量和脑电图的影响。提示脑内积累的DHA对哺乳期新生大鼠脑内催化儿茶酚胺生物合成的酶活性及神经元兴奋性有一定影响.以新生仔猪和断奶仔猪为试验对象，研究了乳铁蛋白经肠腔血液循环进入胆汁的情况。结果表明，乳中的乳铁蛋白主要由肠道进入血液循环，并经胆汁排出体外，提示乳铁蛋白在新生猪体内可能存在肠肝循环。