Analyses of T Cell Proliferation/Differentiation and Repertoire Selection in the thy Usus

T 细胞增殖/分化分析和库选择

基本信息

  • 批准号:
    01480190
  • 负责人:
  • 金额:
    $ 4.35万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1989
  • 资助国家:
    日本
  • 起止时间:
    1989 至 1990
  • 项目状态:
    已结题

项目摘要

A newly established thymic stromal cell clone, MRL104.8a exhibited capacities to express Ia antigens and to support the growth of antigenspecific, interleukin-2 (IL-2) -dependent helper T cell (Th) clones without requirement of antigen and exogenous IL-2. The monolayer of this stromal cell clone exhbited the capacity to maintain immature double-negative thymocytes. Such capacity was also expressed by a factor produced by the MRL104.8a monolayer. This factor designated as Thymic Stroma-derived T cell Growth Factor (TSTGF) was found to be distinct from IL-2 or IL-4 but similar to IL-7 of the previously described cytokines from the functional and molecular aspects. The MRL104.8a monolayer also exerted its defferentiation-promoting effect on double-negative thymocytes. Culture for one day of purified double-negative thymocytes on the monolayer resulted in the induction of an appreciable percent of CD3 4 8 cells. This differentiation could also be induced by a semipurifid TSTGF sample but n … More ot by recombinant IL-7, suggesting that the MRL104.8a cells elaborate a factor (s) responsible for initiating the differentiation of doublenegative cells in addition to the growth-promotion factor identical or closely related to IL-7. When the culture period of double-negative thymocytes was extended to 2 or 3 days, an appreciable number of doublepositive (CD4^+8^+) and single-positive (CD4^+8^-) cells were generated on the MRL104.8a monolayer. The data were also presented that our thymic stromal cells could coutribute to the clonal elimination in the thymus. The growth of a Th clone, 9-16, was supported on the TSTGF-producing and la-expressing MRL104.8a monolayer in the absence of the antigen against which this Th clone is directed. In contrast, the presence ofthe relevant antigen in the MRL104.8a culture elicited the lethal growth-inhibition of Th clone cells. Thus, these observations provide strong support for the proposition that a specialized thymic stromal component plays an essetial role in the intrathymic T cell development in the context of T cell growth and differentiation as well as T cell repertoire selection. Less
一个新建立的胸腺基质细胞克隆,MRL 104.8a表现出表达Ia抗原和支持抗原特异性,白细胞介素-2(IL-2)依赖性辅助T细胞(Th)克隆的生长,而不需要抗原和外源性IL-2的能力。该基质细胞克隆的单层表现出维持未成熟双阴性胸腺细胞的能力。这种能力也由MRL 104.8a单层产生的因子表达。这种被称为胸腺基质衍生T细胞生长因子(TSTGF)的因子被发现不同于IL-2或IL-4,但在功能和分子方面类似于先前描述的细胞因子中的IL-7。MRL 104.8a单层细胞对双阴性胸腺细胞也有分化促进作用。培养一天的纯化的双阴性胸腺细胞的单层导致诱导的CD 3 - 4 - 8细胞的可观的百分比。这种分化也可以被半纯化的TSTGF样品诱导,但不能被诱导。 ...更多信息 而不是重组IL-7,这表明除了与IL-7相同或密切相关的生长促进因子之外,MRL 104.8a细胞还产生了一种负责启动双阴性细胞分化的因子。当双阴性胸腺细胞的培养时间延长到2或3天时,在MRL 104.8a单层上产生了相当数量的双阳性(CD 4 ^+8^+)和单阳性(CD 4 ^+8^-)细胞。实验结果还表明,我们的胸腺基质细胞在胸腺内可参与克隆消除。Th克隆9-16的生长在产生TTGF和表达Ia的MRL 104.8a单层上得到支持,而该Th克隆所针对的抗原不存在。相反,在MRL 104.8a培养物中相关抗原的存在引起Th克隆细胞的致死性生长抑制。因此,这些观察结果提供了强有力的支持的命题,一个专门的胸腺基质成分在胸腺内T细胞发育的T细胞的生长和分化的背景下,以及T细胞库的选择中起着重要的作用。少

项目成果

期刊论文数量(62)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Tatsumi,Y.,et al: "Differentiation of thymocytes from CD3^ー4^ー8^ー through CD3^ー4^ー8^+ into more mature stages induced by a thymic stromal cell clone." Proc.Natl.Acad.Sci.USA.87. 2750-2754 (1990)
Tatsumi,Y.,et al:“胸腺基质细胞克隆诱导胸腺细胞从 CD3^-4^-8^- 到 CD3^-4^-8^+ 分化为更成熟的阶段。” .美国科学.87。2750-2754(1990)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Utsumi,K.,et al.: "Adhesion of immature thymocytes to thymic stromal cells through fibronectin molecules and its significance for the induction of thymocyte differentiation." Proc.Natl.Acad.Sci.USA.
Utsumi,K.,et al.:“未成熟胸腺细胞通过纤连蛋白分子粘附到胸腺基质细胞及其对诱导胸腺细胞分化的意义。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Fujiwara, H., Ogata, M., Mizushima, Y., Tatsumi, Y., Takai, Y., and Hamaoka, T.: "Proliferation and differentiation of immature thymocytes induced by a thymic stromal cell clone" Thymus. 16 :. 159-172 (1990)
Fujiwara, H.、Ogata, M.、Mizushima, Y.、Tatsumi, Y.、Takai, Y. 和 Hamaoka, T.:“胸腺基质细胞克隆诱导的未成熟胸腺细胞的增殖和分化”胸腺。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Oqata, M., Matsubara, H., Takai, Y., Kosaka, H., Katagiri, T., Sano, H., Ishimura, K., Fujita, H., Hamaoka, T., and Fujiwara, H.: "Capacities of a newly established thymic stromal cell clone to express Ia antigens and to produce interleukin 6, colony-stim
Oqata, M.、松原 H.、高井 Y.、小坂 H.、片桐 T.、佐野 H.、石村 K.、藤田 H.、滨冈 T. 和藤原 H.
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Ogata M.et al.: "Capacities of a newly established thymic stromal cell clone to express Ia antigens and to produce interleukin 6,colony-stimulating factor and thymic stroma-derived T cell growth factor." J.Leukocyte Biol.45. 69-78 (1989)
Ogata M.等人:“新建立的胸腺基质细胞克隆表达 Ia 抗原并产生白细胞介素 6、集落刺激因子和胸腺基质衍生 T 细胞生长因子的能力。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

FUJIWARA Hiromi其他文献

FUJIWARA Hiromi的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('FUJIWARA Hiromi', 18)}}的其他基金

Pressure loss mechanism of high fluidity concrete pass through the interspaces of obstacle
高流动性混凝土穿越障碍物间隙压力损失机理
  • 批准号:
    18560447
  • 财政年份:
    2006
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The role of interleukin-12 (IL-12) in T cell activation and IL-12 receptor-mediated signaling
白细胞介素 12 (IL-12) 在 T 细胞激活和 IL-12 受体介导的信号传导中的作用
  • 批准号:
    08457106
  • 财政年份:
    1996
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Tolerance induction of alloreactive T cells and regulation of graft rejection
同种异体反应性 T 细胞的耐受诱导和移植物排斥的调节
  • 批准号:
    04454207
  • 财政年份:
    1992
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Tolerance induction of alloantigen-reactive T cells and analysis of its immunologic and cell-biologic mechanisms
同种异体抗原反应性T细胞的耐受诱导及其免疫学和细胞生物学机制分析
  • 批准号:
    62480165
  • 财政年份:
    1987
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Studies on the analysis of antigen recognition mechanisms of Lyt- <1^+> <2^-> T cells mediating in vivo cell-mediated immunity and the regulation of induction of these cells.
Lyt-<1^><2^->T细胞介导体内细胞介导免疫的抗原识别机制分析及其诱导调节的研究。
  • 批准号:
    60480176
  • 财政年份:
    1985
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了