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Noninvasive quantization of the atherosclerosis by using magnetic resonance imaging

使用磁共振成像对动脉粥样硬化进行无创量化

基本信息

批准号：
01480274
负责人：
KOZUKA Takahiro
金额：
$ 4.22万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

The present study was undertaken to determine whether MN (III) complex of tetrakis (4-sulfonatophenyl) porphyrin (Mn-TSPP) and Mn (III) complex of tetrakis (4-carboxyphenyl) Porphyrin (Mn-TCPP) could be used to reveal the atheroma and sortie tissue in fifteen male New Zealand White rabbits by means of quantitative analysis employing 4.7T MRI and ICP analysis. Compared with control group, the same significant enhancement of noninvolved tissue were demonstrated in both receiving groups (0.050 mmol/kg of MN-TSPP and 0.125 mmol/kg ot' MN-TCPP) at 48 hours after intravenous administration with ex vivo Tl weighted images. Both Mn-complexes failed to enhance the atheromatous lesions. However, ICP analysis revealed the same distribution of manganese between the atheroma and the noninvolved tissues. The atheromatous tissue can be theoretically divided into the extracellular space and the intracellular one. If these agents can distribute into the intracellular space of atheroma, the abundant free-water should commit the enhancement (Tl shortening mechanism). However, MRI results revealed no-enhancement of atheroma. Therefore, MRI and ICP results implicated that Mn-porphyrins distributed into the extracellular space of atheroma. Based on these consideration an appropriate contrast agent depicting atheroselerosis should enter the intracellular space of atheromatous lesions. These characteristics and an adequate combined screening system which consist of MRI and ICP analysis were established in this report.

本文应用4.7T磁共振成像和电感耦合等离子体（ICP）分析技术，对四（4-磺酸基苯基）卟啉锰（Ⅲ）配合物（Mn-TSPP）和四（4-羧基苯基）卟啉锰（Ⅲ）配合物（Mn-TCPP）对15只雄性新西兰白色兔动脉粥样硬化及斑块周围组织的定量分析进行了研究。与对照组相比，静脉注射0.050mmol/kg MN-TSPP和0.125mmol/kg oMN-TCPP后48小时，离体T1加权像显示非受累组织同样显著增强。两种锰络合物均未能增强动脉粥样硬化病变。然而，ICP分析显示在动脉粥样硬化和非受累组织之间锰的分布相同。动脉粥样硬化组织在理论上可分为细胞外间隙和细胞内间隙。如果这些药物能够分布到动脉粥样硬化的细胞内空间，那么丰富的自由水应该会导致增强（T1缩短机制）。然而，MRI结果显示动脉粥样硬化无强化。因此，MRI和ICP结果表明，锰卟啉分布到细胞外间隙的动脉粥样硬化。基于这些考虑，描绘动脉粥样硬化的适当造影剂应该进入动脉粥样硬化病变的细胞内空间。本报告建立了这些特征和适当的联合筛查系统，包括MRI和ICP分析。