Preclinical Study of Radioimmunoguided Surgery Using Metastases Model of Human Colon Cancer in Congenitally Athymic mice.

在先天性无胸腺小鼠中建立了人结肠癌（HCC）在肝、肺和腹膜中的可复制的小鼠转移模型，并评估了使用放射性标记的单克隆抗体（mAb）检测肝转移的γ检测探针（GDP）的效用。腹腔注射后100%出现腹腔转移。此外，60%的小鼠在尾静脉注射后发生肺转移，具有肝转移的小鼠静脉注射40 μ Ci/4 μ g的I-125标记的mAb HT 29-15和同种型匹配的对照mAb（BL-3）。未荷瘤对照组小鼠注射相同剂量的I-125标记的mAb HT 29 -15。使用GDP，每天在心脏区域、肝脏区域（右侧）获得测量值。在具有mAb HT-29-15的荷瘤组中，外部右胁腹/心脏比率显著高于对照组。外部测量允许检测重量仅为161 mg的小肿瘤。在第5天和第7天剖腹手术时获得小的孤立转移的术中测量值。平均转移/心脏GDP比值计数为1.7，可识别重量小至51 mg的肿瘤。这些实验结果证实了GDP用于检测结肠癌小肝转移的有用性，并说明了放射性标记mAb摄取探针测量的重要特征。

Reproducible murine metastases models in the liver, lung, and peritoneum from human colon cancer (HCC) were developed in congenitally athymic mice, and the utility of a gamma detecting probe (GDP) in the detection of hepatic metastases using radiolabeled monoclonal antibody (mAb) was assessed.One hundred percent of mice developed hepatic metastases after intrasplenic injection of HT29LMM HCC cells, and 100% also had peritoneal metastases after intraperitoneal injection. Additionally, 60% of mice developed lung metastases after tail vein injection.Mice with hepatic metastases were injected intravenously with 40 mu Ci/4mugof I-125 labeled mAb HT 29-15 and an isotype-matched control mAb (BL-3). And non-tumor-bearing control mice were injected with the same dose of I-125 labeled mAb HT29-15. Using GDP, measurements were obtained daily over the region of the heart, the region of the liver (the right flank). External right flank/heart ratios were significantly higher in the tumor-bearing group with mAb HT-29-15 than in controls. External measurements allowed detection of small tumors weighing only 161mg. Intraoperative measurements of small isolated metastasis were obtained at laparotomy on days 5 and 7. The mean metastasis/heart GDP ratio count was 1.7, and tumors as small as 51mg in weight could be identified.These experimental results confirm the usefulness of the GDP for the detection of small hepatic metastases from colon cancer, and illustrate important features of probe measurement of radiolabeled mAb uptake.