PRECLINICAL STUDY OF RADIOIMMUNOGUIDED SURGERY FOR COLON CANCER

[导言]我们进行了骨髓移植，以检测骨髓细胞是否具有转分化为胰岛素产生细胞的能力。1×106个骨髓细胞来源于Lac Z转基因大鼠。STZ糖尿病大鼠接受8Gy射线照射，尾静脉注射骨髓细胞。移植8周后，固定胰腺切片，用抗Lac Z、ANITI-NETINE和抗细胞角蛋白19(CK19)抗体染色。巢蛋白是未成熟神经细胞的标志物，CK-19是未成熟胰管细胞在胰腺发育过程中表达的标志物。在胰腺中可见大量细胞同时表达抗Lac Z和抗CK19抗体，而抗Lac Z和抗巢蛋白抗体染色的细胞较少。[目的]探讨抗CK-19抗体阳性的骨髓细胞在胰腺中是否迁移生长，以及在…诱导下是否会分化和再生[方法]用腺相关病毒载体(AAV)克隆了CK启动子/增强子和增强型绿色荧光蛋白(EGFP)基因。将该载体导入骨髓细胞进行培养。1)提取骨髓细胞总RNA，检测ins1、ins2基因的mRNA表达。2)通过尾静脉将骨髓细胞移植给受体大鼠。[结果]1)骨髓细胞表达ins2基因，但不表达ins1、PDX-1基因。2)胰腺腺泡内可见GFP阳性细胞。这些细胞也被抗胰岛素抗体染色，这些结果表明骨髓细胞分化为胰岛素分泌细胞。而胰管内未见GFP阳性细胞。骨髓细胞分化为胰岛素阳性细胞，但没有证据表明骨髓细胞在胰管细胞的发育过程中会再生胰岛素分泌细胞。较少

[Introduction]We performed bone marrow transplantation to examine whether bone marrow cells had ability to trans-differentiate insulin producing cells. 1x10^6 bone marrow cells were derived form Lac Z transgenic rats. STZ diabetic rats were irradiated for eight Gy and received these bone marrow cells by tail vein injection. After eight weeks from transplantation, the section of pancreas was fixed and stained by anti-Lac Z, aniti-nestine and anti-cytokeratin 19(CK19) antibodies. Nestin is a marker of immature neural cells and CK-19 is a marker of immature pancreatic duct cells expressing during the development of pancreas. There were observed many cells that stained by both anti-Lac Z and anti-CK19 antibody in pancreas, but there were few cells that stained by anti-Lac Z and anti-nestine antibody.[Aim]The aim of this study is to elucidate whether anti-CK-19 antibody positive bone marrow cells would migrate and grow in the pancreas, and whether would differentiate and regenerate to insul … More in producing cells.[Method]CK-promoter/enhancer and enhanced green fluorescent protein(EGFP) gene were prepared in cloning site of the adeno-associated virus vector(AAV). Bone marrow cells were transfected this vector and cultured. 1)We extracted total RNA from bone marrow cells and examined the mRNA expression of ins1 and ins2 gene. 2)Bone marrow cells were transplanted to recipient rats via tail vein. We removed the pancreas after eight weeks and stained by anti-insulin antibody.[Results]1)The mRNA expression of ins2 gene was observed in bone marrow cells, but there was no mRNA expression of ins1,pdx-1 gene. 2)GFP positive cells were recognized in acinar glands of pancreas. These cells were also stained by anti-insulin antibody and these results indicated that bone marrow cells differentiated to insulin producing cells. However, there were no GFP positive cells in pancreatic ducts. Bone marrow cells differentiated insulin positive cells, but it was no evidence that bone marrow cells would regenerate insulin producing cells thorough the process of the development in pancreatic duct cells. Less