PRECLINICAL STUDY OF RADIOIMMUNOGUIDED SURGERY FOR COLON CANCER

结肠癌放射免疫引导手术的临床前研究

• 批准号: 14571234
• 负责人: YOSHIDA Kazuhiko
• 金额: $ 2.62万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571234/
• 关键词: colon cancer; monoclonal antibody; radioimmunogudided surgery; 肝転移; radioimmunoguided surgery

[Introduction]We performed bone marrow transplantation to examine whether bone marrow cells had ability to trans-differentiate insulin producing cells. 1x10^6 bone marrow cells were derived form Lac Z transgenic rats. STZ diabetic rats were irradiated for eight Gy and received these bone marrow cells by tail vein injection. After eight weeks from transplantation, the section of pancreas was fixed and stained by anti-Lac Z, aniti-nestine and anti-cytokeratin 19(CK19) antibodies. Nestin is a marker of immature neural cells and CK-19 is a marker of immature pancreatic duct cells expressing during the development of pancreas. There were observed many cells that stained by both anti-Lac Z and anti-CK19 antibody in pancreas, but there were few cells that stained by anti-Lac Z and anti-nestine antibody.[Aim]The aim of this study is to elucidate whether anti-CK-19 antibody positive bone marrow cells would migrate and grow in the pancreas, and whether would differentiate and regenerate to insul … More in producing cells.[Method]CK-promoter/enhancer and enhanced green fluorescent protein(EGFP) gene were prepared in cloning site of the adeno-associated virus vector(AAV). Bone marrow cells were transfected this vector and cultured. 1)We extracted total RNA from bone marrow cells and examined the mRNA expression of ins1 and ins2 gene. 2)Bone marrow cells were transplanted to recipient rats via tail vein. We removed the pancreas after eight weeks and stained by anti-insulin antibody.[Results]1)The mRNA expression of ins2 gene was observed in bone marrow cells, but there was no mRNA expression of ins1,pdx-1 gene. 2)GFP positive cells were recognized in acinar glands of pancreas. These cells were also stained by anti-insulin antibody and these results indicated that bone marrow cells differentiated to insulin producing cells. However, there were no GFP positive cells in pancreatic ducts. Bone marrow cells differentiated insulin positive cells, but it was no evidence that bone marrow cells would regenerate insulin producing cells thorough the process of the development in pancreatic duct cells. Less

[引言]我们进行了骨髓移植，以检查骨髓细胞是否具有转分化胰岛素产生细胞的能力。从Lac Z转基因大鼠中获得1 × 10^6个骨髓细胞。将STZ糖尿病大鼠照射8戈伊，并通过尾静脉注射接受这些骨髓细胞。移植8周后，取胰腺切片，用抗Lac Z、抗巢蛋白和抗细胞角蛋白19（CK 19）抗体染色。Nestin是胰腺发育过程中表达的未成熟神经细胞的标志物，CK-19是胰腺发育过程中表达的未成熟胰管细胞的标志物。在胰腺中观察到许多细胞同时被抗Lac Z和抗CK 19抗体染色，但很少有细胞被抗Lac Z和抗Nestine抗体染色。[目的]探讨抗CK-19抗体阳性的骨髓细胞在胰腺中的迁移和生长，以及在胰腺中的分化和再生， ...更多信息 生产细胞。[方法]在腺相关病毒载体（AAV）的克隆位点制备CK启动子/增强子和增强型绿色荧光蛋白（EGFP）基因。将该载体转染骨髓细胞并培养。1）提取骨髓细胞总RNA，检测ins 1和ins 2基因mRNA的表达。2)骨髓细胞经尾静脉移植到受体大鼠体内。八周后，我们取出胰腺，用抗胰岛素抗体染色。[结果]1）骨髓细胞中有ins 2基因mRNA表达，而ins 1、pdx-1基因mRNA无表达。2)胰腺腺泡内可见GFP阳性细胞。这些细胞也被抗胰岛素抗体染色，这些结果表明骨髓细胞分化为胰岛素产生细胞。胰腺导管内未见GFP阳性细胞。骨髓细胞分化为胰岛素阳性细胞，但没有证据表明骨髓细胞会通过胰管细胞的发育过程再生胰岛素产生细胞。少