Molecular Pathology of Rat Osteosarcoma and Malignant Fibrous Histiocytoma

大鼠骨肉瘤和恶性纤维组织细胞瘤的分子病理学

基本信息

  • 批准号:
    15591596
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2004
  • 项目状态:
    已结题

项目摘要

1)The effect of STI571 was investigated on rat osteosarcoma and MFH cell lines. The cell growth of all cell lines was inhibited in a time- and dose-dependent manner. The inhibition rate were greater for MFH cell lines, about 40%, while about 20% for osteosarcoma cell lines. The difference of effectiveness was depend on PDGFRα expression.2)We investigated the expression and methylation status of p16^<INK4a> gene in rat osteosarcoma and malignant fibrous histiocytoma as well as rat lung adenocarcinoma cell lines. The PCR-SSCP analysis showed no expression of p16 gene in lung adenocarcinoma, while similar expression in ostesarcomas and MFHs as normal fibroblasts. The region close to coding region was hypermethylated in osteosarcoma, in contrast, the region nearby TATA box was consistently methylated in lung adenocarcinoma. MFHs and fibroblasts were hypomethylated in both regions.3)The effect of HMR1275 (flavopiridol) was investigated on rat osteosarcoma, MFH cell lines and lung adenocarcinoma cell lines. The cell growth of all cell lines was inhibited in a time- and dose-dependent manner. The inhibition rate were the greatest for lung adenocarcinoma cell lines, about 80%, while about 60% for osteosarcoma and MFH cell lines. The induction of p16 gene by the treatment of 5-aza 2'-deoxycytidine enhances the effects of HMR1275.4)The effects of 2-methoxyestradiol and histone deacethylase inhibitor were investigated on rat osteosarcoma and MFH cell lines. The cell growth of both cell lines was inhibited in a time- and dose-dependent manner by both 2-methoxyestradiol and histone deacethylase inhibitor. The investigation of the effect mechanisms for these drugs is now on-going including the gene expression such as hypoxia inducible factor, caspase etc.
1)研究STI571对大鼠骨肉瘤和MFH细胞系的影响。所有细胞系的细胞生长均以时间和剂量依赖性方式受到抑制。 MFH细胞系的抑制率较高,约为40%,而骨肉瘤细胞系的抑制率约为20%。疗效的差异取决于PDGFRα的表达。2)我们研究了p16^<INK4a>基因在大鼠骨肉瘤、恶性纤维组织细胞瘤以及大鼠肺腺癌细胞系中的表达和甲基化状态。 PCR-SSCP分析显示p16基因在肺腺癌中不表达,而在骨肉瘤和MFH中与正常成纤维细胞表达相似。骨肉瘤中靠近编码区的区域高度甲基化,相反,肺腺癌中 TATA 框附近的区域持续甲基化。两个区域的MFH和成纤维细胞均呈低甲基化。3)研究了HMR1275(flavopiridol)对大鼠骨肉瘤、MFH细胞系和肺腺癌细胞系的作用。所有细胞系的细胞生长均以时间和剂量依赖性方式受到抑制。对肺腺癌细胞系的抑制率最大,约为80%,对骨肉瘤和MFH细胞系的抑制率约为60%。 5-aza 2'-脱氧胞苷诱导p16基因增强HMR1275的作用。4)研究2-甲氧基雌二醇和组蛋白脱乙酰酶抑制剂对大鼠骨肉瘤和MFH细胞系的影响。 2-甲氧基雌二醇和组蛋白脱乙酰酶抑制剂均以时间和剂量依赖性方式抑制两种细胞系的细胞生长。这些药物作用机制的研究正在进行中,包括缺氧诱导因子、caspase等基因表达。

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Growth inhibition and induction of apoptosis by Flavopiridol in rat lung adenocarcinoma, osteosarcoma and malignant fibrous histiocytoma cell lines.
Flavopiridol 在大鼠肺腺癌、骨肉瘤和恶性纤维组织细胞瘤细胞系中的生长抑制和诱导细胞凋亡。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Honoki K;Yoshitani K;et al.
  • 通讯作者:
    et al.
Growth inhibition of rat osteodsarcoma and malignant fibrous histiocytoma cells by tyrosine kinase inhibitoe STI571.
酪氨酸激酶抑制剂 STI571 对大鼠骨肉瘤和恶性纤维组织细胞瘤细胞的生长抑制。
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Yoshitani;K.;Honoki;K.;et al.
  • 通讯作者:
    et al.
Molecular Genetics in Bone and Soft Tissue Tumors. In ‘New Perspectives in Cancer Research and Therapy' (S.Kuriyama ed.)
骨和软组织肿瘤的分子遗传学。《癌症研究和治疗的新视角》(S.Kuriyama 编辑)
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kanya Honoki;Yoshio Mii
  • 通讯作者:
    Yoshio Mii
Growth Inhibition of rat osteosarcoma and malignant fibrous histiocytoma cells by tyrosine kinase inhibitor STI571
酪氨酸激酶抑制剂STI571对大鼠骨肉瘤和恶性纤维组织细胞瘤细胞生长的抑制作用
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kazuhiro Yoshitani;et al.
  • 通讯作者:
    et al.
Molecular Genetics in Bone and Soft Tissue Tumors. In 'New Perspectives in Cancer Research and Therapy' (S.Kuriyama ed.)
骨和软组织肿瘤的分子遗传学。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kanya Honoki;Yoshio Mii
  • 通讯作者:
    Yoshio Mii
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HONOKI Kanya其他文献

HONOKI Kanya的其他文献

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{{ truncateString('HONOKI Kanya', 18)}}的其他基金

Isolation of cancer stem cells in bone and soft tissue tumors and novel molecular and cellular targeting therapy
骨和软组织肿瘤中癌症干细胞的分离以及新型分子和细胞靶向治疗
  • 批准号:
    20591765
  • 财政年份:
    2008
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Molecular Pathology of Rat Osteosarcoma and Malignant Fibrous Histiocytoma
大鼠骨肉瘤和恶性纤维组织细胞瘤的分子病理学
  • 批准号:
    13671534
  • 财政年份:
    2001
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Histogenesis of malignant fibrous histiocytoma (MFH), with a particular reference to the relationship with mesenchymal differentiation
恶性纤维组织细胞瘤(MFH)的组织发生,特别是与间质分化的关系
  • 批准号:
    09660324
  • 财政年份:
    1997
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
THE HISTOGENESIS OF MALIGNANT FIBROUS HISTIOCYTOMA (MFH), WITH THE SPECIAL REFERENCE TO MULTIPOTENTIAL DIFFERENTIATION OF MFH CELLS
恶性纤维组织细胞瘤(MFH)的组织发生,特别是 MFH 细胞的多向分化
  • 批准号:
    07660424
  • 财政年份:
    1995
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Immunohistochemical Analysis of Pseudomembirane surrrocending mouse DMBA-induced malignant fibrous histiocytoma
假膜周围小鼠 DMBA 诱导的恶性纤维组织细胞瘤的免疫组织化学分析
  • 批准号:
    03670695
  • 财政年份:
    1991
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Histogenesis of Malignant Giant Cell Tumor and Malignant Fibrous Histiocytoma
恶性巨细胞瘤和恶性纤维组织细胞瘤的组织发生
  • 批准号:
    01480367
  • 财政年份:
    1989
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
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