Lectin binding sites in the stria vascularis of normal and endolymphatic hydrops-bearing guinea pigs.

为了阐明豚鼠血管纹边缘细胞的功能，我们进行了以下三个实验：利用凝集素测定特定碳水化合物在Lowicryl K4M包埋材料薄片上的定位。SBA、HPA和RCA-II标记（Gal- nac和Gal）出现在许多根尖囊泡、内体和根尖小管中，以及边缘细胞管腔质膜的微绒毛和微胞浆内陷上。在边缘细胞的基底卷曲的质膜上发现了LFA标记（唾液酸）。WGA （Glu-NAc）沿构成血管纹的所有类型细胞的质膜被检测到。边缘细胞的内化膜、许多顶泡、内体和顶小管具有相同的碳水化合物，这表明这些结构具有相同的碳水化合物成分。可能联系在一起。电镜观察了血管纹边缘细胞的典型囊泡对阳离子化铁蛋白（CF）的摄取。CF颗粒呈簇状，分散在面向内淋巴的自由质膜表面和边缘细胞的小泡中。在根尖囊泡、包被囊泡和核内体中也检测到它们。这些结果表明，顶端囊泡与自由表面质膜有关，并与内淋巴和边缘细胞之间离子的有效交换有关。实验诱导1年后，用细胞化学方法观察了内淋巴液对血管纹na - k - atp酶活性的影响。形态学观察显示边缘细胞的基底褶积细胞间水肿和萎缩，中间细胞萎缩和消失。尽管有这些形态上的改变，但在na - k - atp酶活性的定位方面，在水螅的纹血管和对照标本之间没有发现显著的差异。少

In order to clarify the function of the marginal cells in the guinea pig stria vascularis, the following three experiments were performed :1. Lectins were employed to determine the localization of specific carbohydorates on thin sections of Lowicryl K4M embedded materials. SBA, HPA and RCA-II labeling (Gal-NAc and Gal) was observed in many apical vesicles, endosomes and apical tubules as well as on the microvilli and micropinocytotic invaginations of the luminal plasma membrane of the marginal cells. LFA labeling (sialic acid) was found on the plasma membrane of the basal infoldings of the marginal cells. WGA (Glu-NAc) was detected along the plasma membrane of all types of cells constituting the stria vascularis. The fact that the membranes of internalization, many of the apical vesicles, endosomes and apical tubules in the marginal cells possess the same carbohydrate moieties suggested that these structures. may be associated together.2. The uptake of cationized ferritin (CF) by the a … More pical vesicles in the marginal cells of the stria vascularis was electron microscopically studied. CF particles were found in clusters, scattered on the free surface plasma membrane facing the endolymph and in the caveolae of the marginal cells. They were also detected in the apical vesicles, coated vesicles and endosomes. These findings suggested that the apical vesicles are associated with the free surface plasma membrane, and that they are related to the efficient exchange of ions between the endolymph and the marginal cells.3. The effect of endolymphatic hydrops on the Na-K-ATPase activity in the stria vascularis was cytochemically investigated one year after experimental induction. The morphological observations revealed intercellular dropsy and shrinkage of the basal infoldings of the marginal cells, and shrinkage and disappearance of intermediate cells. In spite of such morphological alterations, no remarkable difference was found, as regards the localization of the Na-K-ATPase activity, between the striae vasculares of hydrops and control specimens. Less