Inhibitory effects of the simultaneous intake of zinc and vitaminsE and C on blood pressure elevation and vascular thickness

摄入膳食抗氧化剂可降低生物体内的氧化应激。DASH（Dietary Stop to Hypertension）建议通过饮食控制来控制血压（BP）。其防治机理可能与蔬菜和水果的抗氧化作用有关，但目前的研究资料还不清楚。即使是维生素E和C，这是最有可能影响血压的抗氧化剂，也没有被证实可以控制血压。由于许多抗氧化营养素相互增强或抵消，我们假设抗氧化营养素的某些组合可能会降低高血压患者的血压升高。为了验证这一假设，我们诱导雄性6周龄Sprague道利大鼠2肾1夹肾血管性高血压，在血压升高后喂食含锌和维生素E和C的饮食，观察血压和血管紧张素（Ang）II的产生。结果发现，单独摄入锌、维生素E或维生素C对高血压模型大鼠的血压没有影响，但三种营养素联合应用可抑制高血压模型大鼠的血压升高，降低血浆AngII水平。在大鼠中，观察到血管壁厚度随着血压升高的抑制而减小。我们还研究了机制，通过染色血管与几个程序。目前，我们正在通过流式细胞仪和荧光显微镜观察中性粒细胞对内皮细胞的影响，从血管内部研究其作用。

Intake of dietary antioxidants reduces oxidative stress in a living body. Dietary Stop to Hypertension（DASH）proposed dietary control of blood pressure (BP) control. The mechanism of the control may involve the antioxidative effects of vegetable and fruits,but the data of the investigations on it are still equivocal. Even vitamins E and C, which were most probable antioxidants to affect BP, were not confirmed to control BP. Since many antioxidative nutrients reinforce or cancel each other, we hypothesized that some combination of antioxidative nutrients may reduce BP elevations in hypertension. To test the hypothesis, we induced 2-kidney, 1-clip renovascular hypertension into male 6 wk Sprague Dawley rats, fed diets containing zinc and vitamins E and C after BP elevation, and observed BP and angiotensin (Ang) II production. As a result, we observed that each sole intake of zinc or vitamin E or C did not affect BP, but the combination of the three nutrients inhibited the BP elevation and decreased plasma AngII level, in the hypertensive model rats. In the rats, the wall thickness of blood vessels was observed to be reduced with the inhibition of BP elevation. We also investigated the mechanism, by staining blood vessels with several procedures. It is now on going to investigate the effects from the inside of vessels by observing the effects of neutrophil on endothelial cells using flow cytometry and fluorescence microscopy.